Crystallization (Comment) | Organism |
---|---|
modeling of 3D structure | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, complete inhibition | Bacillus subtilis | |
PMSF | 5 mM, 60% residual activity, 10 mM, no residual activity | Bacillus subtilis | |
Sodium dodecyl sulfate | 1 mM, 61% resiudal activity | Bacillus subtilis | |
Triton X-100 | 5 mM, 71% residual activity | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, up to 129% stimulation of the protease activity | Bacillus subtilis | |
Fe2+ | 10 mM, up to 112% stimulation of the protease activity | Bacillus subtilis | |
K+ | 10 mM, up to 134% stimulation of the protease activity | Bacillus subtilis | |
Mg2+ | 10 mM, up to 128% stimulation of the protease activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis DR8806 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79.3 | - |
pH 8.0, 37°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | 85% of the activity with casein | Bacillus subtilis | ? | - |
? | |
Bovine serum albumin + H2O | 85% of the activity with casein | Bacillus subtilis DR8806 | ? | - |
? | |
casein + H2O | - |
Bacillus subtilis | ? | - |
? | |
casein + H2O | - |
Bacillus subtilis DR8806 | ? | - |
? | |
Gelatin + H2O | 20% of the activity with casein | Bacillus subtilis | ? | - |
? | |
Gelatin + H2O | 20% of the activity with casein | Bacillus subtilis DR8806 | ? | - |
? | |
N-acetyl-L-tyrosine ethyl ester + H2O | 51% of the activity with casein | Bacillus subtilis | N-acetyl-L-tyrosine + ethanol | - |
? | |
N-acetyl-L-tyrosine ethyl ester + H2O | 51% of the activity with casein | Bacillus subtilis DR8806 | N-acetyl-L-tyrosine + ethanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 85 | at least 50% of maximum activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 70% residual activity | Bacillus subtilis |
70 | - |
1 h, 30% residual activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | isoeletric focusing | - |
6.6 |