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Literature summary for 3.4.21.107 extracted from

  • Jiao, X.D.; Zhang, M.; Cheng, S.; Sun, L.
    Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen (2010), Fish Shellfish Immunol., 28, 672-677.
    View publication on PubMed

Application

Application Comment Organism
medicine purified recombinant DegP is a protective immunogen that can induce the production of specific serum antibodies and elicit strong protective immunity in fish vaccinated with recombinant DegP Edwardsiella tarda

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Edwardsiella tarda

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Edwardsiella tarda
-
-

Organism

Organism UniProt Comment Textmining
Edwardsiella tarda D6BU29 strain TX01
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Edwardsiella tarda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Edwardsiella tarda ?
-
?

Synonyms

Synonyms Comment Organism
DegP
-
Edwardsiella tarda
high temperature requirement A
-
Edwardsiella tarda
HtrA
-
Edwardsiella tarda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Edwardsiella tarda

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50
-
Edwardsiella tarda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant DegP retains 60% of the maximum activity after incubation at 50°C for 1 h Edwardsiella tarda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Edwardsiella tarda

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Edwardsiella tarda

General Information

General Information Comment Organism
physiological function DegP enhances the ability of TX01 to disseminate in fish blood at the advanced stage of infection, heightenes the activity of type 2 autoinducer, and increases the expression of luxS and the genes encoding components of the virulence-associated type III secretion system Edwardsiella tarda