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Literature summary for 3.4.21.107 extracted from

  • Seol, J.H.; Woo, S.K.; Jung, E.M.; Yoo, S.J.; Lee, C.S.; Kim, K.J.; Tanaka, K.; Ichihara, A.; Ha, D.B.; Chung, C.H.
    Protease Do is essential for survival of Escherichia coli at high temperatures: its identity with the htrA gene product (1991), Biochem. Biophys. Res. Commun., 176, 730-736.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
cloned into a Bluescript plasmid and overexpressed in Escherichia coli DH1, disrupted gene transformed to Escherichia coli Jc7623 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information disruption of the ptd gende to obtain lack of activity of protease Do, mutant with prolonged lag period, reduced ability to degrade cell proteins and unable to survive at high temperatures Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strains DH1 and JC7623 (recBc, sbcB)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
HtrA
-
 Escherichia coli
protease do
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 45 no change of cell density of wild type, cell density of the mutant rapidly falls, protease Do essential for the cell's survival at high temperatures Escherichia coli