Literature summary for 3.4.21.106 extracted from

Halabian, R.; Roudkenar, M.H.; Esmaeili, N.S.; Masroori, N.; Roushandeh, A.M.; Najafabadi, A.J.
Establishment of a cell line expressing recombinant factor VII and its subsequent conversion to active form FVIIa through hepsin by genetic engineering method (2009), Vox Sang., 96, 309-315.

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Application

Application	Comment	Organism
biotechnology	the aim of this study is to establish a cell line that directly secrets rFVIIa into cell culture medium: Factor VII and hepsin cDNAs are isolated from HepG2 cell line and cloned into pcDNA3-1 vector. The constructs are co-trasfected to CHO cell line. A cell line that permanently expresses recombinant factor VII (rFVII) and hepsin is established. FVIIa protein is secreted to medium of CHO cells co-transfected with pcNDA3-1-FVII and pcNDA3-1-hepsin. A three- to fourfold decrease in clotting time is observed when human FVII-depleted plasma is used in combination with human thromboplastin in the presence of rFVII, confirming the biological activity of rFVII. A cell line is established expressing FVIIa using genetic engineering methods	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in stably transfected CHO cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Hep-G2 cell	hepsin is isolated from HepG2 cell line	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
hepsin	-	Homo sapiens

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Release 2023.1 (January 2023)