Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | 21% activation at 0.1 mM | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | 94% inhibition at 0.1 mM | Rattus norvegicus | |
diisopropylfluorophosphate | complete inhibition at 5 mM | Rattus norvegicus | |
leupeptin | 99% inhibition at 0.1 mM | Rattus norvegicus | |
phenylmethanesulfonyl fluoride | 80% inhibition at 1 mM | Rattus norvegicus | |
ZnSO4 | 26% inhibition at 1 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions | Rattus norvegicus |
31000 | - |
1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions | Rattus norvegicus |
50000 | - |
zymogen | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Protein + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | zymogen is converted to the active protease by cleavage between Arg161 and Ile162 | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.67 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cleaves after basic amino acid residues, Arg being preferable to Lys | Rattus norvegicus | ? | - |
? | |
N-benzyloxycarbonyl-Ala-Arg-Arg 4-methylcoumarin 7-amide + H2O | weak substrate | Rattus norvegicus | N-benzyloxycarbonyl-Ala-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O | weak substrate | Rattus norvegicus | N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin | - |
? | |
Protein + H2O | - |
Rattus norvegicus | ? | - |
? | |
tert-butyloxycarbonyl-Gln-Ala-Arg 4-methylcoumarin 7-amide + H2O | weak substrate | Rattus norvegicus | tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | weak substrate | Rattus norvegicus | tert-Butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 31000 + 1 * 19000, SDS-PAGE under reducing conditions | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
S01.224 | Merops ID | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity with in the range pH 6-9 has no maximum, the activity increases continuously | Rattus norvegicus |