Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the catalytic transmembrane domain of GlpG into phosphocholine or maltoside detergent micelles of varying alkyl chain lengths. Catalytic turnover numbers depend on detergent alkyl chain length, with saturated chains containing 10-12 carbon atoms supporting maximal activity. Similar results are obtained in phospholipid bicelles, with no proteolytic activity being detected in longer-chain lipids. Hydrophobic mismatch gives rise to a small change in structure and can exert an inhibitory effect on rhomboid activity | Escherichia coli |