Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the soluble cytoplasmic domain, 1.35 Å resolution. The cytoplasmic domain exists as a dimer with extensive domain swapping between the two monomers. Domain-swapped dimers can be isolated from the full-length protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D18A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
E42A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
M3A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
additional information | expression of the isolated membrane domain. Catalytic parameters for the domain are not significantly different in comparison to the full-length protein. Similar to wild-type, membrane domain formsdimers | Escherichia coli |
N33P | mutation promotes domain-swapped dimer formation, due to probably a lower entropic barrier of proteinprotein association | Escherichia coli |
Q14A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
Q30A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
R11A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
R49A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
S68A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
T22A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
W38A | mutation in residue conserved among 32 sequenced prokaryotic rhomboids. No significant change in activity is observed | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
BODIPY FL casein | mutant S68A, pH 8.0, 37°C | Escherichia coli | |
0.00087 | - |
BODIPY FL casein | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.00093 | - |
BODIPY FL casein | mutant R11A, pH 8.0, 37°C | Escherichia coli | |
0.00095 | - |
BODIPY FL casein | mutant D18A, pH 8.0, 37°C | Escherichia coli | |
0.00104 | - |
BODIPY FL casein | mutant Q30A, pH 8.0, 37°C | Escherichia coli | |
0.00104 | - |
BODIPY FL casein | mutant R49A, pH 8.0, 37°C | Escherichia coli | |
0.0012 | - |
BODIPY FL casein | mutant M3A, pH 8.0, 37°C | Escherichia coli | |
0.00126 | - |
BODIPY FL casein | mutant Q14A, pH 8.0, 37°C | Escherichia coli | |
0.00126 | - |
BODIPY FL casein | mutant T22A, pH 8.0, 37°C | Escherichia coli | |
0.00127 | - |
BODIPY FL casein | mutant E42A, pH 8.0, 37°C | Escherichia coli | |
0.00168 | - |
BODIPY FL casein | mutant W38A, pH 8.0, 37°C | Escherichia coli | |
0.0029 | - |
BODIPY FL casein | isolated membrane domain, pH 8.0, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09391 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
BODIPY FL casein + H2O | commercially available fluorescent substrate | Escherichia coli | ? | - |
? | |
additional information | removal of the cytoplasmic domain does not alter the catalytic parameters for detergent-solubilized rhomboid for both substrates BODIPY FL casein and protein TatA | Escherichia coli | ? | - |
? | |
TatA + H2O | - |
Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
BODIPY FL casein | mutant R49A, pH 8.0, 37°C | Escherichia coli | |
0.0038 | - |
BODIPY FL casein | mutant Q30A, pH 8.0, 37°C | Escherichia coli | |
0.0042 | - |
BODIPY FL casein | mutant M3A, pH 8.0, 37°C | Escherichia coli | |
0.0042 | - |
BODIPY FL casein | mutant R11A, pH 8.0, 37°C | Escherichia coli | |
0.0051 | - |
BODIPY FL casein | wild-type, pH 8.0, 37°C | Escherichia coli | |
0.0053 | - |
BODIPY FL casein | mutant Q14A, pH 8.0, 37°C | Escherichia coli | |
0.0058 | - |
BODIPY FL casein | mutant S68A, pH 8.0, 37°C | Escherichia coli | |
0.0059 | - |
BODIPY FL casein | mutant T22A, pH 8.0, 37°C | Escherichia coli | |
0.0069 | - |
BODIPY FL casein | mutant W38A, pH 8.0, 37°C | Escherichia coli | |
0.007 | - |
BODIPY FL casein | isolated membrane domain, pH 8.0, 37°C | Escherichia coli | |
0.0073 | - |
BODIPY FL casein | mutant D18A, pH 8.0, 37°C | Escherichia coli | |
0.0073 | - |
BODIPY FL casein | mutant E42A, pH 8.0, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
BODIPY FL casein | isolated membrane domain, pH 8.0, 37°C | Escherichia coli | |
3.5 | - |
BODIPY FL casein | mutant M3A, pH 8.0, 37°C | Escherichia coli | |
3.67 | - |
BODIPY FL casein | mutant Q30A, pH 8.0, 37°C | Escherichia coli | |
3.67 | - |
BODIPY FL casein | mutant R49A, pH 8.0, 37°C | Escherichia coli | |
4.12 | - |
BODIPY FL casein | mutant W38A, pH 8.0, 37°C | Escherichia coli | |
4.17 | - |
BODIPY FL casein | mutant Q14A, pH 8.0, 37°C | Escherichia coli | |
4.46 | - |
BODIPY FL casein | mutant R11A, pH 8.0, 37°C | Escherichia coli | |
4.63 | - |
BODIPY FL casein | mutant T22A, pH 8.0, 37°C | Escherichia coli | |
5.7 | - |
BODIPY FL casein | mutant E42A, pH 8.0, 37°C | Escherichia coli | |
5.73 | - |
BODIPY FL casein | wild-type, pH 8.0, 37°C | Escherichia coli | |
7.68 | - |
BODIPY FL casein | mutant D18A, pH 8.0, 37°C | Escherichia coli | |
7.78 | - |
BODIPY FL casein | mutant S68A, pH 8.0, 37°C | Escherichia coli |