Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.105 extracted from

  • Vinothkumar, K.R.; Strisovsky, K.; Andreeva, A.; Christova, Y.; Verhelst, S.; Freeman, M.
    The structural basis for catalysis and substrate specificity of a rhomboid protease (2010), EMBO J., 29, 3797-3809.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.105

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 C43(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the truncated GlpG wild type enzyme are obtained by mixing 2.5-3 M ammonium chloride with protein at ratio of 1:1 in hanging drops at 25°C Escherichia coli

Protein Variants

Protein Variants Comment Organism
A253I the mutant exhibits 16% of wild type activity towards the wild type TatA protein but shows 87% of wild type activity on TatA mutant A8G Escherichia coli
A253L the mutant exhibits no activity of wild type activity towards the wild type TatA protein but shows 23% of wild type activity on TatA mutant A8G Escherichia coli
A253T the mutant exhibits 37% of wild type activity towards the wild type TatA protein but shows 63% of wild type activity on TatA mutant A8G Escherichia coli
A253V the mutant exhibits 63% of wild type activity towards the wild type TatA protein but shows 144% of wild type activity on TatA mutant Escherichia coli
H150A the mutation leads to a complete loss of activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
7-amino-4-chloro-3-methoxyisocoumarin
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gurken protein + H2O Escherichia coli
-
 ?
-
 ?
TatA protein + H2O Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09391
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono-Q column chromatography, and Superdex-200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gurken protein + H2O
-
 Escherichia coli ?
-
 ?
TatA protein + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
GlpG
-
 Escherichia coli
rhomboid protease
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.006
-
 in 50 mM HEPES-NaOH (pH 7.5), 0.4 M NaCl, 5 mM EDTA, 10% (v/v) glycerol, and 0.05% (w/v) n-dodecyl-beta-D-maltoside, at 37°C Escherichia coli 7-amino-4-chloro-3-methoxyisocoumarin