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Literature summary for 3.4.21.104 extracted from

  • Duncan, R.C.; Bergstroem, F.; Coetzer, T.H.; Blom, A.M.; Wijeyewickrema, L.C.; Pike, R.N.
    Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4 (2012), Mol. Immunol., 49, 593-600.
    View publication on PubMed
Search for more literature for 3.4.21.104

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21Star(DE3) pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S195A inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-Glu-Gly-L-Arg-chloromethylketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0579
-
 Complement component C4 catalytic MASP-2 fragment CCP1–CCP2–SP, in 0.05 M Tris–HCl, 0.15 M NaCl, 0.2% (w/v) PEG 8000, 0.02% (w/v) NaN3, pH 7.4 at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, MASP-2 CCP1-CCP2-SP form, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GLQRALEI-Lys(Dnp)-NH2 + H2O
-
 Homo sapiens ?
-
 ?
Abz-SLGRKIQI-Lys(Dnp)-NH2 + H2O
-
 Homo sapiens ?
-
 ?
complement component C4 + H2O
-
 Homo sapiens ?
-
 ?
N-tert-butyloxycarbonyl-L-Leu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens tert-butyloxycarbonyl-L-Leu-Gly-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, MASP-2 CCP1-CCP2-SP form, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CCP1-CCP2-SP complement control protein-like domain 1-complement control protein-like domain-2-serine protease, catalytic segment of MASP-2 Homo sapiens
Mannan-binding lectin associated serine protease-2
-
 Homo sapiens
MASP-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.27
-
 Abz-GLQRALEI-Lys(Dnp)-NH2 catalytic MASP-2 fragment CCP1–CCP2–SP, in 0.05 M Tris–HCl, 0.15 M NaCl, 0.2% (w/v) PEG 8000, 0.02% (w/v) NaN3, pH 7.4 at 37°C Homo sapiens
1.92
-
 Abz-SLGRKIQI-Lys(Dnp)-NH2 catalytic MASP-2 fragemt CCP1–CCP2–SP, in 0.05 M Tris–HCl, 0.15 M NaCl, 0.2% (w/v) PEG 8000, 0.02% (w/v) NaN3, pH 7.4 at 37°C Homo sapiens

General Information

General Information Comment Organism
metabolism MASP-2 is the initiating protease of the lectin pathway Homo sapiens