Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C1s(MASP-2CCP1/2) | hybrid C1s/MASP-2 molecule, swapped complement control protein, 21-27fold higher kcat/Km-ratio for complement C4 than C1s(MASP-2SP) | Homo sapiens |
C1s(MASP-2SP) | hybrid C1s/MASP-2 molecule, swapped serine protease, 21-27fold lower kcat/Km-ratio for complement C4 than C1s(MASP-2CCP1/2) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
Complement component C4 | wild-type, 37°C | Homo sapiens | |
0.000147 | - |
Complement component C4 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
0.0028 | - |
Complement component C4 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
0.0052 | - |
Complement component C2 | wild-type, 37°C | Homo sapiens | |
0.0073 | - |
Complement component C2 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
0.0092 | - |
Complement component C2 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
29000 + 60000, chimera C1s(MASP-2SP), SDS-PAGE | Homo sapiens |
78000 | - |
x * 78000, chimera C1s(MASP-2CCP1/2), SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00187 | - |
- |
Purification (Comment) | Organism |
---|---|
chimeric proteins C1s(MASP-2CCP1/2) and C1s(MASP-2SP) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
complement component C2 + H2O | 37°C | Homo sapiens | ? | the complement C3 convertase C4bC2b is generated by cleavage of complement C4 and complement C2 | ? | |
complement component C4 + H2O | 37°C | Homo sapiens | ? | the complement C3 convertase C4bC2b is generated by cleavage of complement C4 and complement C2 | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, chimera C1s(MASP-2CCP1/2), SDS-PAGE | Homo sapiens |
dimer | 29000 + 60000, chimera C1s(MASP-2SP), SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
mannan-binding lectin-associated serine protease-2 | - |
Homo sapiens |
MASP-2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
Complement component C4 | wild-type, 37°C | Homo sapiens | |
2.9 | - |
Complement component C4 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
3.2 | - |
Complement component C4 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
5.1 | - |
Complement component C2 | chimera C1s(MASP-2CCP1/2), 37°C | Homo sapiens | |
5.2 | - |
Complement component C2 | chimera C1s(MASP-2SP), 37°C | Homo sapiens | |
5.6 | - |
Complement component C2 | wild-type, 37°C | Homo sapiens |