Literature summary for 3.4.21.104 extracted from

Harmat, V.; Gal, P.; Kardos, J.; Szilagyi, K.; Ambrus, G.; Vegh, B.; Naray-Szabo, G.; Zavodszky, P.
The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions (2004), J. Mol. Biol., 342, 1533-1546.

Search for more literature for 3.4.21.104

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the catalytic fragment encompassing the second complement control protein module and the serine protease domain, i.e. MASP-2-CCP2-SP, in Escherichia coli strain BL21(DE3), the recombinant construct contains an extra-tetrapeptide ASMT at the N-terminus	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic fragment encompassing the second complement control protein module and the serine protease domain, in presence of Na+, in absence of Mg2+, 0.8 mg/ml purified recombinant protein in 140 mM NaCl, 20 mM Tris-HCl, pH 7.4, and 0.05% w/v NaN3, hanging drop vapour diffusion method at 20°C, mixing with equal volume of reservoir solution containing 30% w/v PEG 6000, 0.2 M NaCl, 10% v/v glycerol, 0.1 M Tris-HCl, pH 7.5, X-ray diffraction structure determination and analysis at 2.25 A resolution	Homo sapiens

Crystallization (Comment)

Organism

catalytic fragment encompassing the second complement control protein module and the serine protease domain, in presence of Na+, in absence of Mg2+, 0.8 mg/ml purified recombinant protein in 140 mM NaCl, 20 mM Tris-HCl, pH 7.4, and 0.05% w/v NaN3, hanging drop vapour diffusion method at 20°C, mixing with equal volume of reservoir solution containing 30% w/v PEG 6000, 0.2 M NaCl, 10% v/v glycerol, 0.1 M Tris-HCl, pH 7.5, X-ray diffraction structure determination and analysis at 2.25 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by Na+	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no induction by Na+	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
complement component C2 + H2O	Homo sapiens	-	2 fragments of complement component C2	-	?
complement component C4 + H2O	Homo sapiens	-	2 fragments of complement C4	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q92876	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	-	Homo sapiens
proteolytic modification	autoactivation	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant catalytic fragment encompassing the second complement control protein module and the serine protease domain, i.e. MASP-2-CCP2-SP, from Escherichia coli strain BL21(DE3)	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
Selective cleavage after Arg223 in complement component C2 (-Ser-Leu-Gly-Arg-/-Lys-Ile-Gln-Ile) and after Arg76 in complement component C4 (-Gly-Leu-Gln-Arg-/-Ala-Leu-Glu-Ile)	structure-function analysis of the catalytic unit, enzyme-substrate interaction is different from enzyme C1 which has the same substrate specificity, MASP-2 shows a subsite flexibility in substrate specificity, catalytic residues are Asp526 and His525, active site structure, overview	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4,4'-dithiopyridine + H2O	-	Homo sapiens	?	-	?
alpha-N-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O	-	Homo sapiens	?	-	?
complement component C2 + H2O	-	Homo sapiens	2 fragments of complement component C2	-	?
complement component C4 + H2O	-	Homo sapiens	2 fragments of complement C4	-	?
complement component C4 + H2O	the complement control protein CCP module is essential for cleavage	Homo sapiens	2 fragments of complement C4	-	?

Subunits

Subunits	Comment	Organism
More	enzyme surface segment analysis, structure-function analysis	Homo sapiens

Synonyms

Synonyms	Comment	Organism
mannose-binding lectin-associated serine protease-2	-	Homo sapiens
MASP-2	-	Homo sapiens
MBL-associated serine protease-2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Homo sapiens