Literature summary for 3.4.21.1 extracted from

Rather, G.M.; Mukherjee, J.; Halling, P.J.; Gupta, M.N.
Activation of alpha chymotrypsin by three phase partitioning is accompanied by aggregation (2012), PLoS ONE, 7, e49241.

Search for more literature for 3.4.21.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	precipitation of alpha-chymotrypsin in the simultaneous presence of ammonium sulfate and tert-butanol, i.e. three phase partitioning, results in preparations which show self aggregation of the enzyme molecules. The aggregates have irregular shapes and have about 3fold higher catalytic activity than the native enzyme. Results indicate that hydrophobic interactions were the driving force behind this aggregation	Bos taurus

Organic Solvent Stability

Organic Solvent	Comment	Organism
tert-Butanol	precipitation of alpha-chymotrypsin in the simultaneous presence of ammonium sulfate and tert-butanol, i.e. three phase partitioning, results in preparations which show self aggregation of the enzyme molecules. The aggregates have irregular shapes and have about 3fold higher catalytic activity than the native enzyme. The aggregates do not differ in lambdamax of fluorescence emission, which is around 340 nm. All the aggregates show higher fluorescence emission intensity. Far-UV and near-UV circular dichroism also show no significant structural changes as compared to the native molecule. HPLC gel filtration gives 14 nm as the diameter for all preparations. Results indicate that hydrophobic interactions were the driving force behind this aggregation	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00766	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bos taurus	-
pancreas	-	Bos taurus	-

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Release 2023.1 (January 2023)