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Literature summary for 3.4.21.1 extracted from

  • Villalba-Villalba, A.G.; Ramirez-Suarez, J.C.; Pacheco-Aguilar, R.; Valenzuela-Soto, E.M.; Lugo-Sanchez, M.E.; Figueroa-Soto, C.G.
    Purification and characterization of chymotrypsin from viscera of vermiculated sailfin catfish, Pterygoplichthys disjunctivus, Weber, 1991 (2013), Fish Physiol. Biochem., 39, 121-130.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, 80% residual activity Pterygoplichthys disjunctivus
EDTA 5 mM, 580.7% residual activity Pterygoplichthys disjunctivus
Fe2+ 5 mM, 73% residual activity Pterygoplichthys disjunctivus
Hg2+ 5 mM, 44% residual activity Pterygoplichthys disjunctivus
NaCl continuous decrease in activity with increasing NaCl concentration, showing 95, 91, 85, 74, 68, and 63% residual activity at 5, 10, 15, 20, 25, and 30% of NaCl, respectively Pterygoplichthys disjunctivus
phenylmethylsulfonyl fluoride 5 mM, 0.7% residual activity Pterygoplichthys disjunctivus
tosyl-L-phenylalanine chloromethyl ketone 5 mM, 5.8% residual activity Pterygoplichthys disjunctivus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
Suc-Ala-Ala-Pro-Phe-4-nitroanilide pH 9.0, 25°C Pterygoplichthys disjunctivus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, SDS-PAGE Pterygoplichthys disjunctivus

Organism

Organism UniProt Comment Textmining
Pterygoplichthys disjunctivus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pterygoplichthys disjunctivus

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
Pterygoplichthys disjunctivus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.4
-
pH 9.0, 25°C Pterygoplichthys disjunctivus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
Pterygoplichthys disjunctivus Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Pterygoplichthys disjunctivus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pterygoplichthys disjunctivus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable below Pterygoplichthys disjunctivus
55
-
labile above Pterygoplichthys disjunctivus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.24
-
Suc-Ala-Ala-Pro-Phe-4-nitroanilide pH 9.0, 25°C Pterygoplichthys disjunctivus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pterygoplichthys disjunctivus

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
88% of maximum activity Pterygoplichthys disjunctivus
10
-
71% of maximum activity Pterygoplichthys disjunctivus

pI Value

Organism Comment pI Value Maximum pI Value
Pterygoplichthys disjunctivus isoelectric focusing
-
3.9

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2670
-
Suc-Ala-Ala-Pro-Phe-4-nitroanilide pH 9.0, 25°C Pterygoplichthys disjunctivus