Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 80% residual activity | Pterygoplichthys disjunctivus | |
EDTA | 5 mM, 580.7% residual activity | Pterygoplichthys disjunctivus | |
Fe2+ | 5 mM, 73% residual activity | Pterygoplichthys disjunctivus | |
Hg2+ | 5 mM, 44% residual activity | Pterygoplichthys disjunctivus | |
NaCl | continuous decrease in activity with increasing NaCl concentration, showing 95, 91, 85, 74, 68, and 63% residual activity at 5, 10, 15, 20, 25, and 30% of NaCl, respectively | Pterygoplichthys disjunctivus | |
phenylmethylsulfonyl fluoride | 5 mM, 0.7% residual activity | Pterygoplichthys disjunctivus | |
tosyl-L-phenylalanine chloromethyl ketone | 5 mM, 5.8% residual activity | Pterygoplichthys disjunctivus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide | pH 9.0, 25°C | Pterygoplichthys disjunctivus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Pterygoplichthys disjunctivus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pterygoplichthys disjunctivus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pterygoplichthys disjunctivus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
viscus | - |
Pterygoplichthys disjunctivus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65.4 | - |
pH 9.0, 25°C | Pterygoplichthys disjunctivus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Pterygoplichthys disjunctivus | Suc-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Pterygoplichthys disjunctivus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pterygoplichthys disjunctivus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable below | Pterygoplichthys disjunctivus |
55 | - |
labile above | Pterygoplichthys disjunctivus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.24 | - |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide | pH 9.0, 25°C | Pterygoplichthys disjunctivus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pterygoplichthys disjunctivus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
88% of maximum activity | Pterygoplichthys disjunctivus |
10 | - |
71% of maximum activity | Pterygoplichthys disjunctivus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pterygoplichthys disjunctivus | isoelectric focusing | - |
3.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2670 | - |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide | pH 9.0, 25°C | Pterygoplichthys disjunctivus |