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Literature summary for 3.4.21.1 extracted from

  • Cunha, E.; Passos, M.L.; Pinto, P.C.; Saraiva, M.L.
    Improved activity of alpha-chymotrypsin in mixed micelles of cetyltrimethylammonium bromide (CTAB) and ionic liquids: a kinetic study resorting to sequential injection analysis (2014), Colloids Surf. B Biointerfaces, 118, 172-178.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.044
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus
0.062
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.065
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
0.109
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.254
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.303
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
0.359
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication Bos taurus
0.375
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information kinetic evaluation of alpha-chymotrypsin activity in cetyltrimethylammonium bromide/ionic liquid mixed micelles and development of an automatic methodology, based on sequential injection analysis. cetyltrimethylammonium bromide/ionic liquids mixed micellar systems can induce alpha-chymotrypsin superactivity. CMC and average micellar size of cetyltrimethylammonium bromide/1-hexyl-3-methylimidazolium chloride, cetyltrimethylammonium bromide/1-butyl-3-methylimidazoliumchloride, cetyltrimethylammonium bromide/1-butyl-1-methylpyrrolidinium and cetyltrimethylammonium bromide/1-butyl-4-methylpyridinium chloride mixed micelles were evaluated by fluorescence and dynamic light scattering, respectively. The sequential injection analysis methodology is suitable for evaluation of alpha-chymotrypsin activity in mixed micelles as it proves to be robust and exhibits good repeatability in all the assay conditions Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00766
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Bos taurus
-
pancreas
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-L-phenylalanine-4-nitroanilide + H2O
-
Bos taurus N-succinyl-L-phenylalanine + 4-nitroaniline
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.154
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.236
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus
0.362
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.417
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
0.48
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.807
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
0.985
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication Bos taurus
1.054
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
1.177
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.74
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication Bos taurus
3.14
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus
3.18
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
3.48
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
4.44
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus
5.35
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication Bos taurus
5.87
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication Bos taurus
6.42
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication Bos taurus
6.47
-
N-succinyl-L-phenylalanine-4-nitroanilide presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication Bos taurus