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Literature summary for 3.4.21.1 extracted from
- Conformational dynamics at the active site of alpha-chymotrypsin and enzymatic activity (2008), Langmuir, 24, 8163-8168.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Proflavin | substrate mimic inhibitor | Bos taurus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.05 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 10°C | Bos taurus | |
| 3.91 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 20°C | Bos taurus | |
| 4.37 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 50°C | Bos taurus | |
| 4.73 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 30°C | Bos taurus | |
| 6.79 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 40°C | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | ? | - |
? | |
| N-acetyl-L-tyrosine ethyl ester + H2O | - |
Bos taurus | N-acetyl-L-tyrosine + ethanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-chymotrypsin | - |
Bos taurus |
| CHT | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
temperature-dependent efficiency of catalysis reveals a bell-shaped feature with a peak at 37°C | Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | catalytic activity of the enzyme exponentially falls off after 40°C and is thought to be associated with the thermal denaturation of the protein, the kcat and KM values associated with the enzyme catalysis increase up to 40°C and then fall | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 10°C | Bos taurus | |
| 4.13 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 20°C | Bos taurus | |
| 8.05 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 50°C | Bos taurus | |
| 12.8 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 30°C | Bos taurus | |
| 21.7 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | 40°C | Bos taurus | |
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Release 2023.1 (January 2023)
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