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Literature summary for 3.4.21.1 extracted from

  • Asgeirsson, B.; Bjarnason, J.B.
    Structural and kinetic properties of chymotrypsin from atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin (1991), Comp. Biochem. Physiol. B, 99B, 327-335.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Gadus morhua
chymostatin
-
Bos taurus
chymostatin
-
Gadus morhua
Cys
-
Gadus morhua
p-tosyl-L-phenylalanine chloromethane
-
Gadus morhua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
N-benzoyl-Tyr p-nitroanilide enzyme form A and enzyme form B, at 25°C Gadus morhua
0.09
-
N-benzoyl-Tyr p-nitroanilide enzyme form A, at 10°C Gadus morhua
0.1
-
N-benzoyl-L-Tyr ethyl ester enzyme form B, at 35°C Gadus morhua
0.11
-
N-benzoyl-Tyr p-nitroanilide enzyme form B, at 35°C Gadus morhua
0.11
-
N-benzoyl-Tyr p-nitroanilide at 10°C Bos taurus
0.11
-
N-benzoyl-Tyr p-nitroanilide enzyme form B, at 10°C Gadus morhua
0.12
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 10°C Gadus morhua
0.14
-
N-benzoyl-Tyr p-nitroanilide at 25°C Bos taurus
0.14
-
N-benzoyl-Tyr p-nitroanilide enzyme form A, at 35°C Gadus morhua
0.14
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 25°C Gadus morhua
0.17
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 35°C Gadus morhua
0.2
-
N-benzoyl-L-Tyr ethyl ester enzyme form B, at 25°C or 10°C Gadus morhua
0.21
-
N-benzoyl-Tyr p-nitroanilide at 35°C Bos taurus
0.24
-
N-benzoyl-L-Tyr ethyl ester at 35°C Bos taurus
0.27
-
N-benzoyl-L-Tyr ethyl ester at 25°C or at 10°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Gadus morhua

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gadus morhua
-
2 enzyme forms: A and B
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
154
-
enzyme form A Gadus morhua
178
-
enzyme form B Gadus morhua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-Tyr ethyl ester + H2O
-
Bos taurus N-benzoyl-L-Tyr + ethanol
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
-
Gadus morhua N-benzoyl-L-Tyr + ethanol
-
?
N-benzoyl-Tyr p-nitroanilide + H2O
-
Bos taurus N-benzoyl-Tyr + p-nitroaniline
-
?
N-benzoyl-Tyr p-nitroanilide + H2O
-
Gadus morhua N-benzoyl-Tyr + p-nitroaniline
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Gadus morhua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information influence of dimethylsulfoxide on the turnover-number Bos taurus
0.05
-
N-benzoyl-Tyr p-nitroanilide at pH 10°C Bos taurus
0.1
-
N-benzoyl-Tyr p-nitroanilide enzyme form A and enzyme form B, at pH 10°C Gadus morhua
0.3
-
N-benzoyl-Tyr p-nitroanilide at 25°C Bos taurus
0.41
-
N-benzoyl-Tyr p-nitroanilide at 35°C Bos taurus
0.41
-
N-benzoyl-Tyr p-nitroanilide enzyme form A, 25°C Gadus morhua
0.42
-
N-benzoyl-Tyr p-nitroanilide enzyme form B, at 25°C Gadus morhua
0.72
-
N-benzoyl-Tyr p-nitroanilide enzyme form B, at 25°C Gadus morhua
0.82
-
N-benzoyl-Tyr p-nitroanilide enzyme form A, at 35°C Gadus morhua
43
-
N-benzoyl-L-Tyr ethyl ester at 10°C Bos taurus
100
-
N-benzoyl-L-Tyr ethyl ester at 35°C Bos taurus
117
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 10°C Gadus morhua
120
-
N-benzoyl-L-Tyr ethyl ester enzyme form B, at 10°C Gadus morhua
142
-
N-benzoyl-L-Tyr ethyl ester at 35°C Bos taurus
207
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 25°C Gadus morhua
214
-
N-benzoyl-L-Tyr ethyl ester enzyme form B, at 32°C Gadus morhua
276
-
N-benzoyl-L-Tyr ethyl ester enzyme form B, at 35°C Gadus morhua
329
-
N-benzoyl-L-Tyr ethyl ester enzyme form A, at 35°C Gadus morhua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
hydrolysis of N-benzoyl-L-Tyr ethyl ester Bos taurus
7.8
-
hydrolysis of N-benzoyl-L-Tyr ethyl ester Gadus morhua

pH Range

pH Minimum pH Maximum Comment Organism
6 9.3 pH 6.0: about 35% of maximal activity, pH 9.3: 40% of maximal activity Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
5°C, about 55% loss of activity after 60 min Gadus morhua
3
-
5°C, about 65% loss of activity after 60 min Gadus morhua
4
-
5°C, about 30% loss of activity after 60 min Gadus morhua
5 12 5°C, stable for 60 min Gadus morhua