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Literature summary for 3.4.19.9 extracted from
- Recombinant zebrafish gamma-glutamyl hydrolase exhibits properties and catalytic activities comparable with those of mammalian enzyme (2009), Drug Metab. Dispos., 37, 302-309.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and inserted into a pET43.1a vector via SmaI and EcoRI sites and expressed in Rosetta (DE3) cells as a Nus-His-tag fusion enzyme (NH-zgammaGH) | Danio rerio |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.057 | - |
5-formyltetrahydrofolate triglutamate | Vmax: 87.6 nmol/min/microg | Danio rerio |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant NH-zgammaGH and zgammaGH appear on SDS-PAGE in the ranges of their estimated size of 92 and 33 kDa, respectively | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Danio rerio | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | ? | - |
? | |
| additional information | Danio rerio AB | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q6NY42 | AB strain | - |
| Danio rerio AB | Q6NY42 | AB strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| after induction with isopropyl thiogalactoside, the enzyme is purified with a Ni-Sepharose column | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyltetrahydrofolate triglutamate + H2O | - |
Danio rerio | ? | - |
? | |
| 5-formyltetrahydrofolate triglutamate + H2O | - |
Danio rerio AB | ? | - |
? | |
| additional information | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | Danio rerio | ? | - |
? | |
| additional information | hydrolysis of the gamma-glutamate bond, resulting in folate-mono-Glu | Danio rerio AB | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | zgammaGH. NH-zgammaGH is a tetramer. It seems that the presence of the N-terminal Nus and His-tag interferes with the quaternary structure of zgammaGH | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-glutamyl hydrolase | - |
Danio rerio |
| gammaGH | - |
Danio rerio |
| zgammaGH | - |
Danio rerio |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.2 | - |
5-formyltetrahydrofolate triglutamate | Vmax: 87.6 nmol/min/microg | Danio rerio | |
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Release 2023.1 (January 2023)
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