Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.9 extracted from

  • Alexander, J.P.; Ryan, T.J.; Ballou, D.P.; Coward, J.K.
    Gamma-glutamyl hydrolase: kinetic characterization of isopeptide hydrolysis using fluorogenic substrates (2008), Biochemistry, 47, 1228-1239.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.9

Activating Compound

Activating Compound Comment Organism Structure
additional information preincubation of the enzyme at 25 °C for 2 h is necessary to obtain maximal activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, detailed steady-state kinetics and partial pre-steady-state kinetics, stopped-flow method, overview Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4,4-difluoro)glutamyl-gamma-glutamate + H2O the fluorine substitution results in a significant decrease in rates of hydrolysis under steady-state conditions due primarily to a 15fold increase in Km compared to the unsubstituted substrate Rattus norvegicus (4,4-difluoro)glutamate + glutamate
-
 ?
(poly-gamma-glutamate)n + H2O
-
 Rattus norvegicus (poly-gamma-glutamate)n-1 + glutamate
-
 ?
4-aminobenzoyl-(4,4-difluoro)glutamyl-gamma-glutamate + H2O the fluorine substitution results in a significant decrease in rates of hydrolysis under steady-state conditions due primarily to a 15fold increase in Km compared to the unsubstituted substrate Rattus norvegicus 4-aminobenzoyl-(4,4-difluoro)glutamate + glutamate
-
 ?
4-aminobenzoyl-gamma-Glu + H2O
-
 Rattus norvegicus 4-aminobenzoate + Glu
-
 ?
4-aminobenzoyl-gamma-Glu-gamma-Glu-Tyr + H2O
-
 Rattus norvegicus ?
-
 ?
glutamyl-gamma-glutamate + H2O
-
 Rattus norvegicus glutamate
-
 ?
additional information either deacylation or rearrangement of the enzyme-product complex is rate-limiting in the isopeptide hydrolysis reaction Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Rattus norvegicus