Cloned (Comment) | Organism |
---|---|
AtGGH1 is expressed in truncated form in Escherichia coli | Arabidopsis thaliana |
AtGGH2 is expressed in truncated form in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
0.00079 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
0.0015 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
0.00186 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Beta vulgaris | 5773 | - |
vacuole | - |
Pisum sativum | 5773 | - |
vacuole | - |
Arabidopsis thaliana | 5773 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O65355 | - |
- |
Beta vulgaris | - |
- |
- |
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant AtGGH1 | Arabidopsis thaliana |
recombinant AtGGH2 | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
root | - |
Beta vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-aminobenzoyl-penta-gamma-glutamate + H2O | AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates | Arabidopsis thaliana | ? | - |
? | |
p-aminobenzoyl-penta-gamma-glutamate + H2O | AtGGH2 cleaves pentaglutamates mainly to monoglutamates | Arabidopsis thaliana | ? | - |
? | |
pteroyl-penta-gamma-glutamate + H2O | AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates | Arabidopsis thaliana | ? | - |
? | |
pteroyl-penta-gamma-glutamate + H2O | AtGGH2 cleaves pentaglutamates mainly monoglutamates | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | AtGGH1 | Arabidopsis thaliana |
dimer | AtGGH2 | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtGGH1 | - |
Arabidopsis thaliana |
AtGGH2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
1.1 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
5.51 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
8.29 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
8.74 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
19.46 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana |