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Literature summary for 3.4.19.9 extracted from

  • Orsomando, G.; de la Garza, R.D.; Green, B.J.; Peng, M.; Rea, P.A.; Ryan, T.J.; Gregory, J.F.; Hanson, A.D.
    Plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles (2005), J. Biol. Chem., 280, 28877-28884.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AtGGH1 is expressed in truncated form in Escherichia coli Arabidopsis thaliana
AtGGH2 is expressed in truncated form in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00052
-
pteroylpentaglutamate 37°C, pH 6.0, AtGGH2 Arabidopsis thaliana
0.00079
-
pteroylpentaglutamate 37°C, pH 6.0, AtGGH1 Arabidopsis thaliana
0.0015
-
p-aminobenzoylpentaglutamate 37°C, pH 6.0, AtGGH1 Arabidopsis thaliana
0.00186
-
p-aminobenzoylpentaglutamate 37°C, pH 6.0, AtGGH2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
Beta vulgaris 5773
-
vacuole
-
Pisum sativum 5773
-
vacuole
-
Arabidopsis thaliana 5773
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O65355
-
-
Beta vulgaris
-
-
-
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AtGGH1 Arabidopsis thaliana
recombinant AtGGH2 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Pisum sativum
-
root
-
Beta vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-aminobenzoyl-penta-gamma-glutamate + H2O AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates Arabidopsis thaliana ?
-
?
p-aminobenzoyl-penta-gamma-glutamate + H2O AtGGH2 cleaves pentaglutamates mainly to monoglutamates Arabidopsis thaliana ?
-
?
pteroyl-penta-gamma-glutamate + H2O AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates Arabidopsis thaliana ?
-
?
pteroyl-penta-gamma-glutamate + H2O AtGGH2 cleaves pentaglutamates mainly monoglutamates Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
dimer AtGGH1 Arabidopsis thaliana
dimer AtGGH2 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtGGH1
-
Arabidopsis thaliana
AtGGH2
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 pteroylpentaglutamate 37°C, pH 6.0, AtGGH2 Arabidopsis thaliana
1.1
-
pteroylpentaglutamate 37°C, pH 6.0, AtGGH1 Arabidopsis thaliana
5.51
-
p-aminobenzoylpentaglutamate 37°C, pH 6.0, AtGGH2 Arabidopsis thaliana
8.29
-
p-aminobenzoylpentaglutamate 37°C, pH 6.0, AtGGH1 Arabidopsis thaliana
8.74
-
pteroylpentaglutamate 37°C, pH 6.0, AtGGH2 Arabidopsis thaliana
19.46
-
pteroylpentaglutamate 37°C, pH 6.0, AtGGH1 Arabidopsis thaliana