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Literature summary for 3.4.19.9 extracted from

  • Rhee, M.S.; Lindau-Shepard, B.; Chave, K.J.; Galivan, J.; Ryan, T.J.
    Characterization of human cellular gamma-glutamyl hydrolase (1998), Mol. Pharmacol., 53, 1040-1046.
    View publication on PubMed
Search for more literature for 3.4.19.9

Cloned(Commentary)

Cloned (Comment) Organism
expression with a baculovirus/Sf9 insect cell expression system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate inhibition of wild-type enzyme and mutant enzymes C19A, C124A and C290A Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
baculovirus-expressed enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methotrexate diglutamate + H2O poor substrate Homo sapiens ?
-
 ?
methotrexate hexaglutamate + H2O
-
 Homo sapiens methotrexate pentaglutamate + methotrexate tetraglutamate + methotrexate triglutamate + methotrexate diglutamate + methotrexate monoglutamate
-
 ?
methotrexate pentaglutamate + H2O
-
 Homo sapiens methotrexate tetraglutamate + methotrexate triglutamate + methotrexate diglutamate + methotrexate monoglutamate + L-glutamate
-
 ?
methotrexate tetraglutamate + H2O
-
 Homo sapiens ?
-
 ?
methotrexate triglutamate + H2O
-
 Homo sapiens methotrexate monoglutamate + methotrexate diglutamate + glutamate
-
 ?
additional information the maximal velocity decreases in the order methothrexate hexaglutamate, methotrexate tetraglutamate, methotrexate pentaglutamate, methotrexate triglutamate Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Homo sapiens