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Literature summary for 3.4.19.9 extracted from
- gamma-Glutamyl hydrolase from pea cotyledons (1993), Phytochemistry, 32, 1109-1117.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| heparin | no inhibition | Pisum sativum |  |
| additional information | no inhibition by 2-mercaptomethylglutaric acid | Pisum sativum | |
| p-hydroxymercuribenzoate | - |
Pisum sativum | |
| Phenylmethylsulfonylfluoride | - |
Pisum sativum | |
| pteroylpentaglutamate | hydrolysis of pteroyltriglutamate | Pisum sativum | |
| Zn2+ | - |
Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
Pteroyltriglutamate | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | mainly, only minor amounts in the vacuolar and mitochondrial fraction | Pisum sativum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
cv. Homesteader | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | six-day-old | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-aminobenzoyltriglutamate + H2O | at 13% the rate of pteroyltriglutamate hydrolysis | Pisum sativum | ? | - |
? | |
| additional information | no hydrolysis of gamma-glutamyl tripeptide | Pisum sativum | ? | - |
? | |
| additional information | no hydrolysis of N-carbobenzoxyphenylalanyl-alanine | Pisum sativum | ? | - |
? | |
| polyglutamylfolate + H2O | - |
Pisum sativum | ? | - |
? | |
| pteroylpentaglutamate + H2O | - |
Pisum sativum | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Pisum sativum | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Pisum sativum | pteroylglutamate + glutamate | + pteroyldiglutamate | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
hydrolysis of pteroyltriglutamate and pteroylpentaglutamate | Pisum sativum |
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Release 2023.1 (January 2023)
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