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Literature summary for 3.4.19.6 extracted from
- Purification of and kinetic studies on a narrow specificity synaptosomal membrane pyroglutamate aminopeptidase from guinea-pig brain (1985), Eur. J. Biochem., 150, 47-52.
General Stability
| General Stability | Organism |
|---|---|
| inclusion of 50% glycerol prevents most of the harmful effect of freezing in imidazole buffer | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Luliberin | inhibits hydrolysis of thyroliberin competitively | Cavia porcellus |  |
| Peptides containing N-terminal pyroglutamyl residues | - |
Cavia porcellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
Thyroliberin | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| synaptosome | - |
Cavia porcellus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3237 | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, potassium phosphate buffer, pH 7.4, stable for 6 months | Cavia porcellus |
| 15°C, 5% loss of activity after 7 days | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acid-thyroliberin + H2O | - |
Cavia porcellus | ? | - |
? | |
| luliberin N-terminal tripeptide + H2O | pyroglutamyl-His-Trp | Cavia porcellus | ? | - |
? | |
| additional information | no hydrolysis of pyroglutamyl peptides containing more than three amino acids | Cavia porcellus | ? | - |
? | |
| additional information | no hydrolysis of dipeptides containing N-terminal pyroglutamic acid | Cavia porcellus | ? | - |
? | |
| pyroglutamyl-His-Gly + H2O | slight | Cavia porcellus | pyroglutamate + His-Gly | - |
? | |
| thyroliberin + H2O | - |
Cavia porcellus | pyroglutamic acid + His-Pro-NH2 | - |
? | |
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