Literature summary for 3.4.19.6 extracted from

Chavez-Gutierrez, L.; Bourdais, J.; Aranda, G.; Vargas, M.A.; Matta-Camacho, E.; Ducancel, F.; Segovia, L.; Joseph-Bravo, P.; Charli, J.L.
A truncated isoform of pyroglutamyl aminopeptidase II produced by exon extension has dominant-negative activity (2005), J. Neurochem., 92, 807-817.

Cloned(Commentary)

Cloned (Comment)	Organism
detection of a RNA species derived from an alternative processing at the exon 14intron 14 boundary. The alternatively processed RNA encodes a shorter version of PPII (PPII), lacking part of the C-terminal domain. PPII is expressed in COS-7 (or C6 glioma) cells but it does not exhibit any PPII activity. Co-transfection of PPII and increasing amounts of PPII* expression vectors results in a dose-dependent reduction in PPII activity and the formation of covalent PPIIPPII* heterodimers. PPII* is therefore a powerful dominant-negative isoform of PPII, and heterodimerization may be its mechanism of action. Natural expression of shortened versions of M1 aminopeptidases may constitute a new mode of regulation of their activity	Rattus norvegicus

Cloned (Comment)

Organism

detection of a RNA species derived from an alternative processing at the exon 14intron 14 boundary. The alternatively processed RNA encodes a shorter version of PPII (PPII*), lacking part of the C-terminal domain. PPII* is expressed in COS-7 (or C6 glioma) cells but it does not exhibit any PPII activity. Co-transfection of PPII and increasing amounts of PPII* expression vectors results in a dose-dependent reduction in PPII activity and the formation of covalent PPIIPPII* heterodimers. PPII* is therefore a powerful dominant-negative isoform of PPII, and heterodimerization may be its mechanism of action. Natural expression of shortened versions of M1 aminopeptidases may constitute a new mode of regulation of their activity

Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
additional information	detection of a RNA species derived from an alternative processing at the exon 14intron 14 boundary. The alternatively processed RNA encodes a shorter version of PPII (PPII), lacking part of the C-terminal domain. PPII is expressed in COS-7 (or C6 glioma) cells but it does not exhibit any PPII activity. Co-transfection of PPII and increasing amounts of PPII* expression vectors results in a dose-dependent reduction in PPII activity and the formation of covalent PPIIPPII* heterodimers. PPII* is therefore a powerful dominant-negative isoform of PPII, and heterodimerization may be its mechanism of action. Natural expression of shortened versions of M1 aminopeptidases may constitute a new mode of regulation of their activity	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	detection of a RNA species derived from an alternative processing at the exon 14intron 14 boundary. The alternatively processed RNA encodes a shorter version of PPII (PPII), lacking part of the C-terminal domain. PPII is expressed in COS-7 (or C6 glioma) cells but it does not exhibit any PPII activity. Co-transfection of PPII and increasing amounts of PPII* expression vectors results in a dose-dependent reduction in PPII activity and the formation of covalent PPIIPPII* heterodimers. PPII* is therefore a powerful dominant-negative isoform of PPII, and heterodimerization may be its mechanism of action. Natural expression of shortened versions of M1 aminopeptidases may constitute a new mode of regulation of their activity	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	detection of a RNA species derived from an alternative processing at the exon 14intron 14 boundary. The alternatively processed RNA encodes a shorter version of PPII (PPII), lacking part of the C-terminal domain. PPII is expressed in COS-7 (or C6 glioma) cells but it does not exhibit any PPII activity. Co-transfection of PPII and increasing amounts of PPII* expression vectors results in a dose-dependent reduction in PPII activity and the formation of covalent PPIIPPII* heterodimers. PPII* is therefore a powerful dominant-negative isoform of PPII, and heterodimerization may be its mechanism of action. Natural expression of shortened versions of M1 aminopeptidases may constitute a new mode of regulation of their activity	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
PPII	-	Rattus norvegicus