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Literature summary for 3.4.19.6 extracted from

  • Chavez-Gutierrez, L.; Matta-Camacho, E.; Osuna, J.; Horjales, E.; Joseph-Bravo, P.; Maigret, B.; Charli, J.L.
    Homology modeling and site-directed mutagenesis of pyroglutamyl peptidase II. Insights into omega-versus aminopeptidase specificity in the M1 family (2006), J. Biol. Chem., 281, 18581-18590.
    View publication on PubMed
Search for more literature for 3.4.19.6

Protein Variants

Protein Variants Comment Organism
K463N mutant losses omega-peptidase but displays alanyl-aminopeptidase activity Rattus norvegicus
S269A mutant shows a 40% decrease in activity with thyrotropin-releasing hormone-beta-naphthylamide as substrate Rattus norvegicus
S269E mutant is inactive with thyrotropin-releasing hormone-beta-naphthylamide as substrate Rattus norvegicus
S269Q mutant is inactive with thyrotropin-releasing hormone-beta-naphthylamide as substrate Rattus norvegicus
S269Q/K463N mutant is inactive with thyrotropin-releasing hormone-beta-naphthylamide as substrate Rattus norvegicus
S269Q/K463R mutant is inactive with thyrotropin-releasing hormone-beta-naphthylamide as substrate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00679
-
 thyrotropin-releasing hormone-beta-naphthylamide mutant enzyme S269A Rattus norvegicus
0.00942
-
 thyrotropin-releasing hormone-beta-naphthylamide wild-type enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Rattus norvegicus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression of wild-type and mutant enzymes in COS-7 cells and C6 glioma Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thyrotropin-releasing hormone-beta-naphthylamide + H2O essential role of Glu408 within the exopeptidase motif for aminopeptidase activity. Ser269 and Lys463 are implicated in the generation of omegapeptidase specificity. Lys463 creates a putative salt bridge with Glu408 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
PPII
-
 Rattus norvegicus