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Literature summary for 3.4.19.6 extracted from

  • Bauer, K.
    Purification and characterization of the thyrotropin-releasing-hormone-degrading ectoenzyme (1994), Eur. J. Biochem., 224, 387-396.
    View publication on PubMed
Search for more literature for 3.4.19.6

General Stability

General Stability Organism
after one cycle of freezing at -80°C and thawing, the activity of the purified enzyme preparation containing 0.3, 0.1, 0.03 or 0.01 mg protein/ml decreases by 18%, 36%, 73% and 99%, respectively Sus scrofa
below a critical protein concentration of 1 mg/ml the enzyme is sensitive to freezing and thawing Sus scrofa
presence of bovine serum albumin, 1%, sucrose, glycerol or trehalose prevents inactivation during freezing and thawing Sus scrofa
unstable in Tris/HCl buffer, pH 7.3 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
5-oxoproline product inhibition at high concentration Sus scrofa
Chelating agents
-
 Sus scrofa
His-Pro-NH2 product inhibition at high concentration Sus scrofa
additional information no inhibition by chlordiazepoxide; no inhibition by DTNB; no inhibition by NEM; no inhibition by pepstatin; no inhition by 2-iodoacetamide Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 thyrotropin-releasing hormone
-
 Sus scrofa
0.248
-
 5-oxoprolyl 2-naphthylamide
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
membrane
-
 Sus scrofa 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 2 * 116000, SDS-PAGE Sus scrofa
230000
-
-
 Rattus norvegicus
230000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus
glycoprotein
-
 Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus
additional information
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
stable at 4°C for more than 2 months or for 24 h at 25°C, in phosphate or diethylmalonate buffer, pH 7.3, irrespective of the protein concentration Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5-oxoprolyl 2-naphthylamide + H2O very poor substrate Rattus norvegicus 2-naphthylamine + 5-oxoproline
-
 ?
L-5-oxoprolyl 2-naphthylamide + H2O very poor substrate Sus scrofa 2-naphthylamine + 5-oxoproline
-
 ?
thyroliberin + H2O i.e. pyroglutamyl-His-Pro-NH2, thyrotropin-releasing hormone Rattus norvegicus pyroglutamic acid + His-Pro-NH2
-
 ?
thyroliberin + H2O i.e. pyroglutamyl-His-Pro-NH2, thyrotropin-releasing hormone Sus scrofa pyroglutamic acid + His-Pro-NH2
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus
dimer 2 * 116000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 5-oxoprolyl 2-naphthylamide
-
 Sus scrofa
38.3
-
 thyrotropin-releasing hormone
-
 Sus scrofa