Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T168A | mutant does not show enzymatic activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14550 | - |
mass spectroscopy, beta-subunit | Homo sapiens |
18000 | - |
Western blot, reducing conditions, detected in soluble and insoluble fractions | Homo sapiens |
18500 | - |
mass spectroscopy, alpha-subunit | Homo sapiens |
33000 | - |
Western blot, reducing conditions, detected in soluble and insoluble fractions | Homo sapiens |
33020 | - |
mass spectroscopy, precursor enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q7L266 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography. hASRGL1 purification by immobilized metal ion affinity chromatography yields 30 mg of protein/l with a purity of more than 90% as determined by SDS-PAGE | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-L-Asp-L-Ala + H2O | - |
Homo sapiens | L-Asp + L-Ala | - |
? | |
beta-L-Asp-L-Leu + H2O | - |
Homo sapiens | L-Asp + L-Leu | - |
? | |
beta-L-Asp-L-Lys + H2O | - |
Homo sapiens | L-Asp + L-Lys | - |
? | |
beta-L-Asp-L-Phe + H2O | - |
Homo sapiens | L-Asp + L-Phe | - |
? | |
beta-L-Asp-L-Phe methyl ester + H2O | - |
Homo sapiens | L-Asp + L-Phe methyl ester | - |
? | |
L-Asp beta-methyl ester + H2O | - |
Homo sapiens | L-Asp + methanol | - |
? | |
L-aspartic acid beta-(7-amido-4-methylcoumarin) + H2O | - |
Homo sapiens | L-aspartic acid + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
asparaginase-like protein 1 | - |
Homo sapiens |
hASRGL1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |