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Literature summary for 3.4.19.3 extracted from
- Purification and characterization of L-pyrrolidonecarboxylate peptidase from Bacillus amyloliquefaciens (1978), J. Biochem., 84, 467-476.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol and EDTA stabilize | Bacillus amyloliquefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | CdCl2 | Bacillus amyloliquefaciens |  |
| Cu2+ | CuCl2 | Bacillus amyloliquefaciens | |
| DFP | - |
Bacillus amyloliquefaciens | |
| Hg2+ | HgCl2 | Bacillus amyloliquefaciens | |
| L-pyroglutamate | weak | Bacillus amyloliquefaciens | |
| N-bromosuccinimide | - |
Bacillus amyloliquefaciens | |
| Ni2+ | NiSO4, weak | Bacillus amyloliquefaciens | |
| PCMB | - |
Bacillus amyloliquefaciens | |
| PCMB | - |
Niallia circulans | |
| Sodium tetrathionate | - |
Bacillus amyloliquefaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
| FeCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
| FeCl3 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
| MgCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
| MnCl2 | 1 mM, weak activation | Bacillus amyloliquefaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
| 72000 | - |
gel filtration | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus subtilis | - |
strain PCI 219, ML-208 and strain DT-39 | - |
| Bacillus subtilis | - |
strain mito, strain NSI and strain tamarii | - |
| Micrococcus luteus | - |
- |
- |
| Niallia circulans | - |
- |
- |
| Paenibacillus polymyxa | - |
- |
- |
| Pimelobacter simplex | - |
- |
- |
| Priestia megaterium | - |
- |
- |
| Proteus vulgaris | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus amyloliquefaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus amyloliquefaciens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, stable for 3 months | Bacillus amyloliquefaciens |
| 4°C, pH 7, in presence of 5 mM EDTA and 5 mM 2-mercaptoethanol, less than 50% loss of activity | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyroglutamyl-4-methylumbelliferyl ester + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
| pyroglutamyl-Ala + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + Ala | - |
? | |
| pyroglutamyl-beta-naphthylamide + H2O | - |
Bacillus subtilis | pyroglutamate + beta-naphthylamine | - |
? | |
| pyroglutamyl-D-Ala + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + D-Ala | - |
? | |
| pyroglutamyl-D-Leu + H2O | - |
Bacillus amyloliquefaciens | pyroglutamate + D-Leu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 24000, SDS-PAGE | Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus amyloliquefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable | Bacillus amyloliquefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Bacillus amyloliquefaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | pH 5.5: about 50% of maximal activity, pH 10.0: about 65% of maximal activity | Bacillus amyloliquefaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | 30°C, stable in presence of 5 mM EDTA and 2-mercaptoethanol | Bacillus amyloliquefaciens |
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