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Literature summary for 3.4.19.3 extracted from

  • Hellstroem, J.L.; Vehniaeinen, M.; Mustonen, M.; Loevgren, T.; Lamminmaeki, U.; Hellman, J.
    Unfolding of the immunoglobulin light and heavy chains is required for the enzymatic removal of N-terminal pyroglutamyl residues (2006), Biochim. Biophys. Acta, 1764, 1735-1740.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Pyrococcus furiosus
methanol a methanol based deblocking solution preserves enzymatic activity, provides conditions compatible with sequencing and enhances deblocking of electroblotted samples Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
PGAP expressed from a pET3a-series vector in Escherichia coli BL21(DE) cells Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by cation exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-pyroglutamyl p-nitroanilide + H2O
-
Pyrococcus furiosus L-pyroglutamate + p-nitroaniline
-
?
additional information PGAP performs enzymatic deblocking of pyroglutamylated immunoglobulins. Unfolding of immunoglobulins is required for excision of the pyroglutamate. The enzyme requires the substrate in an extended conformation, which is not fullfiled in the native form of immunoglobulins Pyrococcus furiosus ?
-
?

Synonyms

Synonyms Comment Organism
PGAP
-
Pyrococcus furiosus
pyroglutamate aminopeptidase
-
Pyrococcus furiosus