Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Pyrococcus furiosus | |
methanol | a methanol based deblocking solution preserves enzymatic activity, provides conditions compatible with sequencing and enhances deblocking of electroblotted samples | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
PGAP expressed from a pET3a-series vector in Escherichia coli BL21(DE) cells | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by cation exchange chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-pyroglutamyl p-nitroanilide + H2O | - |
Pyrococcus furiosus | L-pyroglutamate + p-nitroaniline | - |
? | |
additional information | PGAP performs enzymatic deblocking of pyroglutamylated immunoglobulins. Unfolding of immunoglobulins is required for excision of the pyroglutamate. The enzyme requires the substrate in an extended conformation, which is not fullfiled in the native form of immunoglobulins | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGAP | - |
Pyrococcus furiosus |
pyroglutamate aminopeptidase | - |
Pyrococcus furiosus |