Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Bacillus licheniformis |
General Stability | Organism |
---|---|
mutant enzyme R109M shows high salt stability with almost no loss in activity even in the presence of 20% (w/v) NaCl whereas wild-type enzyme and mutant enzyme R109K retain only 50% of residual activity | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | A9YTT0 | - |
- |
Bacillus licheniformis ER-15 | A9YTT0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gamma-glutamyl-p-nitroanilide + H2O | hydrolytic activity | Bacillus licheniformis | L-glutamate + p-nitroaniline | - |
? | |
additional information | the enzyme also shows transpeptidase activity | Bacillus licheniformis | ? | - |
? | |
additional information | the enzyme also shows transpeptidase activity | Bacillus licheniformis ER-15 | ? | - |
? | |
additional information | the enzyme also shows transpeptidase activity | Bacillus licheniformis ER15 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000 + x * 22000, SDS-PAGE, all the enzyme variants are of the same molecular weight with large subunit located around 45 kDa and small subunit at 22 kDa | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
optimum of wild-type enzyme, mutant enzyme R109K and R109M | Bacillus licheniformis |
70 | - |
optimum of mutant enzyme R109S | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
t1/2: 176 min (mutant enzyme R109M), 16.5 min (mutant enzyme R109S), 69.3 min (wild-type enzyme), 19.8 min (mutant enzyme R109K) | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
pH-optimum of mutant enzyme R109M | Bacillus licheniformis |
9 | - |
comparable activities at both pH 9.0 and pH 10.0. A similar pattern is observed for R109S. Mutant enzyme R109K shows a sharp pH-optimum at pH 9.0 | Bacillus licheniformis |
10 | - |
comparable activities at both pH 9.0 and pH 10.0. A similar pattern is observed for R109S | Bacillus licheniformis |