Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
A587S | no hydrolytic activity | Sus scrofa |
N675S | no hydrolytic activity | Sus scrofa |
Y707H | no hydrolytic activity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
N-acetyl-Met-Ala | wild-type enzyme | Sus scrofa | |
0.56 | - |
N-acetyl-Met-Ala | recombinant wild-type enzyme | Sus scrofa | |
0.68 | - |
N-acetyl-Met-Ala | mutant enzyme D562N | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Met-Ala + H2O | - |
Sus scrofa | N-acetyl-Met + Ala | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.4 | - |
N-acetyl-Met-Ala | mutant enzyme D562N | Sus scrofa | |
29 | - |
N-acetyl-Met-Ala | recombinant wild-type enzyme | Sus scrofa | |
33 | - |
N-acetyl-Met-Ala | wild-type enzyme | Sus scrofa |