Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.1 extracted from

  • Kobayashi, K.; Smith, J.A.
    Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction (1987), J. Biol. Chem., 262, 11435-11445.
    View publication on PubMed

Application

Application Comment Organism
analysis enzyme is useful for deblocking of peptides Rattus norvegicus

General Stability

General Stability Organism
75% loss of activity in 0.1 M guanidine hydrochloride at 37°C after 200 min Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3,3'-dimethylaminopropylcarbodiimide
-
Rattus norvegicus
5,5'-dithiobis-(2-nitrobenzoate) partial Rattus norvegicus
Cl- activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala Rattus norvegicus
DFP
-
Rattus norvegicus
diethyl dicarbonate
-
Rattus norvegicus
Gly methyl ester
-
Rattus norvegicus
iodoacetamide partial Rattus norvegicus
iodoacetic acid partial Rattus norvegicus
N-acetyl-Ala competitive Rattus norvegicus
N-acetyl-Ala chloromethyl ketone inactivation follows first order kinetics, acetyl-Ala protects Rattus norvegicus
N-ethyl-5-phenylisoxazolium 3'-sulfonate i.e. Woodward's reagent Rattus norvegicus
SCN- activity towards N-acetyl-Ala-Ala and N-acetyl-Ala-Ala-Ala Rattus norvegicus
Zn2+ most potent inhibitor Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- 0.1-0.5 M, activates activity towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide Rattus norvegicus
SCN- 0.1-0.5 M, activates towards N-acetyl-Ala p-nitroanilide and N-acetyl-Ala beta-naphthylamide Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
290000 320000 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Ala p-nitroanilide + H2O
-
Rattus norvegicus N-acetyl-Ala + p-nitroaniline
-
?
N-acetyl-Ala-Ala + H2O
-
Rattus norvegicus N-acetyl-Ala + Ala
-
?
N-acetyl-Ala-Ala-Ala + H2O
-
Rattus norvegicus N-acetyl-Ala + Ala-Ala
-
?
N-acetyl-Ala-Asp + H2O
-
Rattus norvegicus N-acetyl-Ala + Asp
-
?
N-acetyl-Ala-beta-naphthylamide + H2O
-
Rattus norvegicus N-acetyl-Ala + beta-naphthylamine
-
?
N-acetyl-Ala-Gly + H2O
-
Rattus norvegicus N-acetyl-Ala + Gly
-
?
N-acetyl-Ala-Leu + H2O
-
Rattus norvegicus N-acetyl-Ala + Leu
-
?
N-acetyl-Ala-Met + H2O
-
Rattus norvegicus N-acetyl-Ala + Met
-
?
N-acetyl-Ala-Phe + H2O
-
Rattus norvegicus N-acetyl-Ala + Phe
-
?
N-acetyl-Ala-Ser + H2O
-
Rattus norvegicus N-acetyl-Ala + Ser
-
?
N-acetyl-Ala-Thr + H2O
-
Rattus norvegicus N-acetyl-Ala + Thr
-
?
N-acetyl-Ala-Trp + H2O
-
Rattus norvegicus N-acetyl-Ala + Trp
-
?
N-acetyl-Ala-Tyr + H2O
-
Rattus norvegicus N-acetyl-Ala + Tyr
-
?
N-acetyl-Gly-Ala + H2O weak activity Rattus norvegicus N-acetyl-Gly + Ala
-
?
N-acetyl-Leu-Ala + H2O
-
Rattus norvegicus N-acetyl-Leu + Ala
-
?
N-acetyl-Met-Ala + H2O
-
Rattus norvegicus N-acetyl-Met + Ala
-
?
N-acetyl-Phe-Ala + H2O weak activity Rattus norvegicus N-acetyl-Phe + Ala
-
?
N-acetyl-Ser-Ala + H2O
-
Rattus norvegicus N-acetyl-Ser + Ala
-
?
N-acetyl-Tyr-Ala + H2O weak activity Rattus norvegicus N-acetyl-Tyr + Ala
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
hydrolysis of N-acetyl-Ala p-nitroanilide Rattus norvegicus