Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.1 extracted from

  • Li, R.; Zhang, F.; Cao, S.-G.; Xie, G.-Q.; Gao, R.J.
    Expression and characterization of a thermostable acyl-peptide releasing enzyme ST0779 from Sulfolobus tokodaii (2012), Chem. Res. Chin. Univ., 28, 851-855.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 10% inhibition Sulfurisphaera tokodaii
K+ 1 mM, 4% inhibition Sulfurisphaera tokodaii
SDS 1 mg/ml, complete inhibition Sulfurisphaera tokodaii
Triton X-100 1 mg/ml, 4% inhibition Sulfurisphaera tokodaii
Tween 20 1 mg/ml, 10% inhibition Sulfurisphaera tokodaii
Tween 80 1 mg/ml, 18% inhibition Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q973W9
-
-
Sulfurisphaera tokodaii 7 Q973W9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala + H2O
-
Sulfurisphaera tokodaii Ala + Ala
-
?
Ala-Ala + H2O
-
Sulfurisphaera tokodaii 7 Ala + Ala
-
?
Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii Ala + Ala-Ala
-
?
Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii 7 Ala + Ala-Ala
-
?
Ala-Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii Ala + Ala-Ala-Ala
-
?
formyl-Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii formyl-Ala + Ala-Ala
-
?
formyl-Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii 7 formyl-Ala + Ala-Ala
-
?
N-acetyl-Ala-Ala + H2O
-
Sulfurisphaera tokodaii N-acetyl-Ala + Ala
-
?
N-acetyl-Ala-Ala + H2O
-
Sulfurisphaera tokodaii 7 N-acetyl-Ala + Ala
-
?
N-acetyl-Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii N-acetyl-Ala + Ala-Ala
-
?
N-acetyl-Ala-Ala-Ala + H2O
-
Sulfurisphaera tokodaii 7 N-acetyl-Ala + Ala-Ala
-
?

Synonyms

Synonyms Comment Organism
AARE
-
Sulfurisphaera tokodaii
acyl-peptide releasing enzyme
-
Sulfurisphaera tokodaii
ST0779
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
substrate: N-acetyl-Ala-Ala-Ala Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
55 h, 70% of the activioty remains Sulfurisphaera tokodaii
90
-
half-life: 16 h Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
substrate: N-acetyl-Ala-Ala-Ala Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 65% of maximal activity, pH 9.5: about 70% of maximal activity Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 25°C, 24 h, over 85% activity remaining Sulfurisphaera tokodaii