Cloned (Comment) | Organism |
---|---|
into the vector pET11a for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells | Aeropyrum pernix K1 |
Protein Variants | Comment | Organism |
---|---|---|
D15A | mutant to determine the effects of the N-terminal region and the salt bridges on the stability and catalytic activity of apAPH | Aeropyrum pernix K1 |
D15A/R18A | mutant to determine the effects of the N-terminal region and the salt bridges on the stability and catalytic activity of apAPH | Aeropyrum pernix K1 |
DELTAN21 | mutant, N-terminal helix deleted, no longer functional at the optimum temperature, 95°C, for the wild-type enzyme, low thermodynamic stability | Aeropyrum pernix K1 |
R18A | mutant to determine the effects of the N-terminal region and the salt bridges on the stability and catalytic activity of apAPH | Aeropyrum pernix K1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00913 | - |
Ac-Leu-p-nitroanilide | mutant D15A | Aeropyrum pernix K1 | |
0.0094 | - |
Ac-Leu-p-nitroanilide | mutant R18A | Aeropyrum pernix K1 | |
0.00958 | - |
Ac-Leu-p-nitroanilide | mutant DELTAN21 | Aeropyrum pernix K1 | |
0.00963 | - |
Ac-Leu-p-nitroanilide | mutant D15A/R18A | Aeropyrum pernix K1 | |
0.01038 | - |
Ac-Leu-p-nitroanilide | wild-type | Aeropyrum pernix K1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeropyrum pernix K1 | removal of an N-acylated amino acid from blocked peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix K1 | Q9YBQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
using Hi-Trap Q-Sepharose and HiLoad Sephacryl S-200 columns | Aeropyrum pernix K1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Leu-p-nitroanilide + H2O | substrate peptidase assay | Aeropyrum pernix K1 | Ac-Leu + p-nitroaniline | - |
? | |
additional information | removal of an N-acylated amino acid from blocked peptides | Aeropyrum pernix K1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
Aeropyrum pernix K1 |
apAPH | - |
Aeropyrum pernix K1 |
APH | - |
Aeropyrum pernix K1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
mutant DELTAN21 | Aeropyrum pernix K1 |
92 | - |
mutant D15A above 92° | Aeropyrum pernix K1 |
92 | - |
mutant R18A above 92° | Aeropyrum pernix K1 |
95 | - |
wild-type enzyme | Aeropyrum pernix K1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.04 | - |
Ac-Leu-p-nitroanilide | wild-type | Aeropyrum pernix K1 | |
2.1 | - |
Ac-Leu-p-nitroanilide | mutant R18A | Aeropyrum pernix K1 | |
2.14 | - |
Ac-Leu-p-nitroanilide | mutant D15A/R18A | Aeropyrum pernix K1 | |
2.21 | - |
Ac-Leu-p-nitroanilide | mutant D15A | Aeropyrum pernix K1 | |
2.64 | - |
Ac-Leu-p-nitroanilide | mutant DELTAN21 | Aeropyrum pernix K1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Aeropyrum pernix K1 |