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Literature summary for 3.4.19.1 extracted from

  • Yamin, R.; Bagchi, S.; Hildebrant, R.; Scaloni, A.; Widom, R.L.; Abraham, C.R.
    Acyl peptide hydrolase, a serine proteinase isolated from conditioned medium of neuroblastoma cells, degrades the amyloid-beta peptide (2007), J. Neurochem., 100, 458-467.
    View publication on PubMed

Application

Application Comment Organism
medicine pharmaceutical target for the treatment of Alzheimer’s disease Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into the TOPO-V5/His tag mammalian expression vector. Wild-type and mutant APH constructs transfected into COS-7 or SK-N-MC cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S587A mutated in the active site Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-butane boronic acid
-
Homo sapiens
4-(2-aminoethyl) benzenesulfonyl fluoride
-
Homo sapiens
Ac-Ala competitive inhibitor Homo sapiens
Ac-Leu-CH2-Cl irreversible inhibitor Homo sapiens
Ac-Met competitive inhibitor Homo sapiens
diisopropyl fluorophosphate
-
Homo sapiens
additional information alphabeta peptide (1-40) can inhibit APH activity from the cell lysates of APH transfected cells after IP at 0.01 and 0.001 mM concentration, while reversed alphabeta peptide (40-1) at the same concentrations does not show any inhibitory effect Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
APH isolated from COS-7 cells purified on a nickel column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain APH expression in Alzheimer’s disease brain is lower than in age-matched controls Homo sapiens
-
neuroblastoma cell isolated from serum-free conditioned medium Homo sapiens
-
SK-N-MC cell isolated from serum-free conditioned medium Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid-beta peptide + H2O
-
Homo sapiens ?
-
?
N-acetyl-alanyl-4-nitroanilide + H2O
-
Homo sapiens N-acetyl-L-Ala + 4-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
acyl peptide hydrolase
-
Homo sapiens
APH
-
Homo sapiens