Application | Comment | Organism |
---|---|---|
medicine | pharmaceutical target for the treatment of Alzheimers disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the TOPO-V5/His tag mammalian expression vector. Wild-type and mutant APH constructs transfected into COS-7 or SK-N-MC cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S587A | mutated in the active site | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butane boronic acid | - |
Homo sapiens | |
4-(2-aminoethyl) benzenesulfonyl fluoride | - |
Homo sapiens | |
Ac-Ala | competitive inhibitor | Homo sapiens | |
Ac-Leu-CH2-Cl | irreversible inhibitor | Homo sapiens | |
Ac-Met | competitive inhibitor | Homo sapiens | |
diisopropyl fluorophosphate | - |
Homo sapiens | |
additional information | alphabeta peptide (1-40) can inhibit APH activity from the cell lysates of APH transfected cells after IP at 0.01 and 0.001 mM concentration, while reversed alphabeta peptide (40-1) at the same concentrations does not show any inhibitory effect | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
APH isolated from COS-7 cells purified on a nickel column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | APH expression in Alzheimers disease brain is lower than in age-matched controls | Homo sapiens | - |
neuroblastoma cell | isolated from serum-free conditioned medium | Homo sapiens | - |
SK-N-MC cell | isolated from serum-free conditioned medium | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid-beta peptide + H2O | - |
Homo sapiens | ? | - |
? | |
N-acetyl-alanyl-4-nitroanilide + H2O | - |
Homo sapiens | N-acetyl-L-Ala + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl peptide hydrolase | - |
Homo sapiens |
APH | - |
Homo sapiens |