Protein Variants | Comment | Organism |
---|---|---|
R526 I | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 17.3fold higher than the wild-type ratio | Aeropyrum pernix |
R526A | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 11.7fold higher than the wild-type ratio | Aeropyrum pernix |
R526E | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 115.5fold higher than the wild-type ratio | Aeropyrum pernix |
R526K | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 13.9fold higher than the wild-type ratio | Aeropyrum pernix |
R526L | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 14.8fold higher than the wild-type ratio | Aeropyrum pernix |
R526V | the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 22.3fold higher than the wild-type ratio | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, wild-type enzyme | Aeropyrum pernix | |
1.3 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526L | Aeropyrum pernix | |
1.7 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526V | Aeropyrum pernix | |
2.1 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526I | Aeropyrum pernix | |
4.3 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526A | Aeropyrum pernix | |
5.8 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526E | Aeropyrum pernix | |
10.5 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526K | Aeropyrum pernix | |
35.7 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526V | Aeropyrum pernix | |
38.2 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526K | Aeropyrum pernix | |
38.2 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526L | Aeropyrum pernix | |
40.1 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526I | Aeropyrum pernix | |
41.6 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526A | Aeropyrum pernix | |
43.3 | - |
p-nitrophenyl caprylate | 80°C, wild-type enzyme | Aeropyrum pernix | |
114.6 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526E | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YBQ2 | K1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Leu-p-nitroanilide + H2O | esterase activity of wild-type enzyme with p-nitrophenyl caprylate as substrate is 7times higher than peptidase activity with N-acetyl-Leu-p-nitroanilide as substrate, 150fold higher for mutant enzyme R526V, peptidase activity for mutant R526E is abolished | Aeropyrum pernix | N-acetyl-Leu + p-nitroaniline | - |
? | |
p-nitrophenyl caprylate + H2O | esterase activity of wild-type enzyme with p-nitrophenyl caprylate as substrate is 7times higher than peptidase activity with N-acetyl-Leu-p-nitroanilide as substrate, 150fold higher for mutant enzyme R526V, peptidase activity for mutant R526E is abolished | Aeropyrum pernix | nitrophenol + caprylate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526E | Aeropyrum pernix | |
6.6 | - |
p-nitrophenyl caprylate | 80°C, wild-type enzyme | Aeropyrum pernix | |
8.1 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526E | Aeropyrum pernix | |
9.2 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526I | Aeropyrum pernix | |
9.3 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, wild-type enzyme | Aeropyrum pernix | |
9.4 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526K | Aeropyrum pernix | |
9.6 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526A | Aeropyrum pernix | |
9.6 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526L | Aeropyrum pernix | |
9.7 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526V | Aeropyrum pernix | |
12.6 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526A | Aeropyrum pernix | |
20.8 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526I | Aeropyrum pernix | |
26.9 | - |
N-acetyl-Leu-p-nitroanilide | 80°C, mutant enzyme R526K | Aeropyrum pernix | |
28.5 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526L | Aeropyrum pernix | |
30.9 | - |
p-nitrophenyl caprylate | 80°C, mutant enzyme R526V | Aeropyrum pernix |