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Literature summary for 3.4.19.1 extracted from

  • Wang, Q.; Yang, G.; Liu, Y.; Feng, Y.
    Discrimination of esterase and peptidase activities of acylaminoacyl peptidase from hyperthermophilic Aeropyrum pernix K1 by a single mutation (2006), J. Biol. Chem., 281, 18618-18625.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
R526 I the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 17.3fold higher than the wild-type ratio Aeropyrum pernix
R526A the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 11.7fold higher than the wild-type ratio Aeropyrum pernix
R526E the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 115.5fold higher than the wild-type ratio Aeropyrum pernix
R526K the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 13.9fold higher than the wild-type ratio Aeropyrum pernix
R526L the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 14.8fold higher than the wild-type ratio Aeropyrum pernix
R526V the ratio of kcat/Km for p-nitrophenyl caprylate to kcat/KM for N-acetyl-Leu-p-nitroanilide is 22.3fold higher than the wild-type ratio Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
N-acetyl-Leu-p-nitroanilide 80°C, wild-type enzyme Aeropyrum pernix
1.3
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526L Aeropyrum pernix
1.7
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526V Aeropyrum pernix
2.1
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526I Aeropyrum pernix
4.3
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526A Aeropyrum pernix
5.8
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526E Aeropyrum pernix
10.5
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526K Aeropyrum pernix
35.7
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526V Aeropyrum pernix
38.2
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526K Aeropyrum pernix
38.2
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526L Aeropyrum pernix
40.1
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526I Aeropyrum pernix
41.6
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526A Aeropyrum pernix
43.3
-
p-nitrophenyl caprylate 80°C, wild-type enzyme Aeropyrum pernix
114.6
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526E Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YBQ2 K1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Leu-p-nitroanilide + H2O esterase activity of wild-type enzyme with p-nitrophenyl caprylate as substrate is 7times higher than peptidase activity with N-acetyl-Leu-p-nitroanilide as substrate, 150fold higher for mutant enzyme R526V, peptidase activity for mutant R526E is abolished Aeropyrum pernix N-acetyl-Leu + p-nitroaniline
-
?
p-nitrophenyl caprylate + H2O esterase activity of wild-type enzyme with p-nitrophenyl caprylate as substrate is 7times higher than peptidase activity with N-acetyl-Leu-p-nitroanilide as substrate, 150fold higher for mutant enzyme R526V, peptidase activity for mutant R526E is abolished Aeropyrum pernix nitrophenol + caprylate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526E Aeropyrum pernix
6.6
-
p-nitrophenyl caprylate 80°C, wild-type enzyme Aeropyrum pernix
8.1
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526E Aeropyrum pernix
9.2
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526I Aeropyrum pernix
9.3
-
N-acetyl-Leu-p-nitroanilide 80°C, wild-type enzyme Aeropyrum pernix
9.4
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526K Aeropyrum pernix
9.6
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526A Aeropyrum pernix
9.6
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526L Aeropyrum pernix
9.7
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526V Aeropyrum pernix
12.6
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526A Aeropyrum pernix
20.8
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526I Aeropyrum pernix
26.9
-
N-acetyl-Leu-p-nitroanilide 80°C, mutant enzyme R526K Aeropyrum pernix
28.5
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526L Aeropyrum pernix
30.9
-
p-nitrophenyl caprylate 80°C, mutant enzyme R526V Aeropyrum pernix