Literature summary for 3.4.18.1 extracted from

Edge, M.; Forder, C.; Hennam, J.; Lee, I.; Tonge, D.; Hardern, I.; Fitton, J.; Eckersley, K.; East, S.; Shufflebotham, A.; Blakey, D.; Slater, A.
Engineered human carboxypeptidase B enzymes that hydrolyse hippuryl-L-glutamic acid: reversed-polarity mutants (1998), Protein Eng., 11, 1229-1234.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of recombinants in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D253K	reversed polarity and improved activity	Homo sapiens
D253R	reversed polarity and improved activity	Homo sapiens
G251T/D253K	double mutant, improved activity	Homo sapiens
additional information	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.69	-	hippuryl-L-Arg	D253R mutant	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hippuryl-L-Arg + H2O	-	Homo sapiens	Hippuric acid + L-Arg	-	?
hippuryl-L-glutamic acid + H2O	-	Homo sapiens	hippuric acid + L-Glu	-	?

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Release 2023.1 (January 2023)