Literature summary for 3.4.17.B1 extracted from

Occhipinti, E.; Bec, N.; Gambirasio, B.; Baietta, G.; Martelli, P.L.; Casadio, R.; Balny, C.; Lange, R.; Tortora, P.
Pressure and temperature as tools for investigating the role of individual non-covalent interactions in enzymatic reactions Sulfolobus solfataricus carboxypeptidase as a model enzyme (2006), Biochim. Biophys. Acta, 1764, 563-572.

Search for more literature for 3.4.17.B1

Application

Application	Comment	Organism
additional information	effect of temperature and pressure on conformational modifications and enzyme-substrate interactions are generally dissimilar and barely predictable	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as an N-terminus His-tagged protein in Escherichia coli	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	L-Bz-His	at pH 8.0 and 35°C	Saccharolobus solfataricus
0.013	-	L-Fur-Phe	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.014	-	L-Bz-Ala	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.014	-	L-Bz-Arg	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.019	-	L-Bz-Glu	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.021	-	L-Bz-His	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.028	-	L-Bz-Met	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.028	-	Fur-Gly	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.034	-	L-Bz-Phe	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.043	-	L-Bz-Phe	at pH 8.0 and 35°C	Saccharolobus solfataricus
0.061	-	Bz-Gly	at pH 8.0 and 35°C	Saccharolobus solfataricus
0.103	-	L-Fur-Leu	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.143	-	Bz-Gly	at pH 6.5 and 35°C	Saccharolobus solfataricus
0.144	-	L-Bz-Phe	at pH 5.7 and 35°C	Saccharolobus solfataricus
0.149	-	Bz-Gly	at pH 5.7 and 35°C	Saccharolobus solfataricus
0.181	-	L-Bz-His	at pH 5.7 and 35°C	Saccharolobus solfataricus
0.501	-	L-Bz-Leu	at pH 6.5 and 35°C	Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by affinity chromatography	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Bz-Gly + H2O	-	Saccharolobus solfataricus	benzoic acid + glycine	-	?
Fur-Gly + H2O	-	Saccharolobus solfataricus	furoic acid + glycine	-	?
L-Bz-Ala + H2O	-	Saccharolobus solfataricus	benzoic acid + L-alanine	-	?
L-Bz-Arg + H2O	-	Saccharolobus solfataricus	benzoic acid + L-arginine	-	?
L-Bz-Glu + H2O	-	Saccharolobus solfataricus	benzoic acid + L-glutamate	-	?
L-Bz-His + H2O	-	Saccharolobus solfataricus	benzoic acid + L-histidine	-	?
L-Bz-Leu + H2O	-	Saccharolobus solfataricus	benzoic acid + L-leucine	-	?
L-Bz-Met + H2O	-	Saccharolobus solfataricus	benzoic acid + L-methionine	-	?
L-Bz-Phe + H2O	-	Saccharolobus solfataricus	benzoic acid + L-phenylalanine	-	?
L-Fur-Leu + H2O	-	Saccharolobus solfataricus	furoic acid + L-leucine	-	?
L-Fur-Phe + H2O	-	Saccharolobus solfataricus	furoic acid + L-phenylalanine	-	?

Synonyms

Synonyms	Comment	Organism
CPS	-	Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	65	-	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.03	-	Bz-Gly	at pH 5.7 and 35°C	Saccharolobus solfataricus
1.65	-	Bz-Gly	at pH 8.0 and 35°C	Saccharolobus solfataricus
2.05	-	L-Bz-His	at pH 8.0 and 35°C	Saccharolobus solfataricus
2.3	-	Fur-Gly	at pH 6.5 and 35°C	Saccharolobus solfataricus
2.8	-	L-Bz-Ala	at pH 6.5 and 35°C	Saccharolobus solfataricus
2.95	-	L-Bz-Met	at pH 6.5 and 35°C	Saccharolobus solfataricus
3.25	-	L-Fur-Phe	at pH 6.5 and 35°C	Saccharolobus solfataricus
3.58	-	L-Fur-Leu	at pH 6.5 and 35°C	Saccharolobus solfataricus
3.63	-	L-Bz-Arg	at pH 6.5 and 35°C	Saccharolobus solfataricus
4.15	-	Bz-Gly	at pH 6.5 and 35°C	Saccharolobus solfataricus
4.3	-	L-Bz-Glu	at pH 6.5 and 35°C	Saccharolobus solfataricus
4.3	-	L-Bz-Phe	at pH 8.0 and 35°C	Saccharolobus solfataricus
4.57	-	L-Bz-Phe	at pH 5.7 and 35°C	Saccharolobus solfataricus
4.87	-	L-Bz-Phe	at pH 6.5 and 35°C	Saccharolobus solfataricus
7.77	-	L-Bz-His	at pH 6.5 and 35°C	Saccharolobus solfataricus
8.35	-	L-Bz-Leu	at pH 6.5 and 35°C	Saccharolobus solfataricus
8.83	-	L-Bz-His	at pH 5.7 and 35°C	Saccharolobus solfataricus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.7	8	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)