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Literature summary for 3.4.17.4 extracted from

  • Felix, F.; Brouillet, N.
    Purification et proprietes de deux peptidases de levure de brasserie (1966), Biochim. Biophys. Acta, 122, 127-144.
    View publication on PubMed
Search for more literature for 3.4.17.4

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline Ki: 0.035 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+ Saccharomyces cerevisiae
Ag+ complete inhibition at a concentration of 1 mM Saccharomyces cerevisiae
diisopropylphosphorofluoridate no inhibition Saccharomyces cerevisiae
EDTA Ki: 0.013 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+, Zn2+ and Co2+, at a concentration of 0.1 mM, are most effecient with 88% and 62% reactivation Saccharomyces cerevisiae
iodoacetamide 90% inhibition at a concentration of 5 mM Saccharomyces cerevisiae
iodoacetate 40% inhibition at a concentration of 5 mM, irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol Saccharomyces cerevisiae
p-chloromercuribenzoate irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can reverse the inhibition by chelating agents Saccharomyces cerevisiae
Co2+ can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 62%, inhibits the enzyme at higher concentrations, 10 mM Saccharomyces cerevisiae
Mn2+ can reverse the inhibition by chelating agents Saccharomyces cerevisiae
Zn2+ can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 88%, inhibits the enzyme at higher concentrations, 10 mM Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Purification (Commentary)

Purification (Comment) Organism
400fold Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
230
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Gly-Gly + H2O 7.2% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae ?
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 ?
benzoyl-Gly-Lys + H2O 2% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae ?
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 ?
benzyloxycarbonyl-Ala-Arg(NO2) + H2O 21% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Ala + nitroarginine
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 ?
Benzyloxycarbonyl-Ala-Phe + H2O 10% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae Benzyloxycarbonyl-Ala + Phe
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 ?
Benzyloxycarbonyl-Glu-Tyr + H2O 1.1% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae Benzyloxycarbonyl-Glu + Tyr
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 ?
benzyloxycarbonyl-Gly-Glu + H2O 31% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Gly + Glu
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 ?
benzyloxycarbonyl-Gly-Gly + H2O 13% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Gly + Gly
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 ?
Benzyloxycarbonyl-Gly-Leu + H2O best substrate Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Leu
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 ?
Benzyloxycarbonyl-Gly-Phe + H2O 31% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae Benzyloxycarbonyl-Gly + Phe
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 ?
benzyloxycarbonyl-Phe-Leu + H2O 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
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 ?
benzyloxycarbonyl-Phe-Met + H2O 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Met
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 ?
benzyloxycarbonyl-Phe-Phe + H2O 1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Phe
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 ?
dibenzyloxycarbonyl-Lys-Leu + H2O 5.2% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae dibenzyloxycarbonyl-Lys + Leu
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 ?
Gly-Leu-Gly + H2O 3% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae ?
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 ?
Gly-Leu-Tyr + H2O 12% of the activity compared to benzyloxycarbonyl-Gly-Leu Saccharomyces cerevisiae ?
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 ?
additional information no hydrolysis of benzyloxycarbonyl-Leu-Leu, benzyloxycarbonyl-Leu-Phe, dibenzyloxycarbonyl-Lys-Phe, benzyloxycarbonyl-Phe-Ser, benzyloxycarbonyl-His-Leu, benzyloxycarbonyl-Pro-Try, benzyloxycarbonyl-Gly-Gly-Leu, benzyloxycarbonyl-Gly-Gly-Phe, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzyloxycarbonyl-Ala-Phe-NH2, benzyloxycarbonyl-Phe-Leu-NH2, benzyloxycarbonyl-Phe-Leu-NH2 and Gly-Leu Saccharomyces cerevisiae ?
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 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.2 benzyloxycarbonyl-Gly-Leu as substrate, in presence of 0.5 M NaCl Saccharomyces cerevisiae