Literature summary for 3.4.17.22 extracted from

Sidyelyeva, G.; Baker, N.E.; Fricker, L.D.
Characterization of the molecular basis of the Drosophila mutations in carboxypeptidase D. Effect on enzyme activity and expression (2006), J. Biol. Chem., 281, 13844-13852.

Search for more literature for 3.4.17.22

Application

Application	Comment	Organism
additional information	Drosophila CPD is encoded by the silver gene svr, which is differentially spliced to produce long transmembrane protein forms with three metallocarboxypeptidase-like domains and short soluble forms with a single metallocarboxypeptidase domain. Flies that retain the short form are viable, whereas flies that are missing all forms of CPD do not survive past the early larval stages	Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment)	Organism
baculovirus expression of constructs containing either the 1A or the 1B form and one of three different sequences of domain 2 of CPD (wild type, mutants svr1, or svrpoi) in Sf9 cells	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
additional information	svrPG33 mutants, do not survive past the early larval stage. These mutants have a P-element insertion within exon 1B upstream of the initiation ATG, which greatly reduces mRNA levels of all forms of CPD. Both svr1 and svrpoi mutants are viable, both deletions eliminate enzyme activity of the second CP-like domain and appear to cause the misfolding of the protein. This greatly reduces the levels of the long forms of CPD protein but do not affect the levels of the short forms	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Drosophila melanogaster	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	antisera raised against the N-terminal region of the 1A form and the C-terminal region of the tail-2 form of CPD	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	P42787	-	-

Purification (Commentary)

Purification (Comment)	Organism
on affinity resin	Drosophila melanogaster

Synonyms

Synonyms	Comment	Organism
Carboxypeptidase D	-	Drosophila melanogaster
CPD	-	Drosophila melanogaster

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Release 2023.1 (January 2023)