Cloned (Comment) | Organism |
---|---|
expression in Drosophila S2 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
X-ray structures of seven complexes of the hydrolytically inactive recombinant E424A mutant and polyglutamylated folates substrates, refined at resolutions of between 1.65 and 2.00 A, and mutant H475Y, in complex with substrate N-acetyl-L-aspartyl-L-glutamate to 1.83 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E424A | catalytically inactive, series of X-ray structures of complexes a panel of naturally occurring polyglutamylated folates | Homo sapiens |
H475Y | X-ray structure in complex with substrate N-acetyl-L-aspartyl-L-glutamate,wild-type and the H475 variant are functionally identical. both proteins share an almost identical arrangement of amino acids in the vicinity of residue 475, and the imidazole ring of wild-type His475 and the benzene ring of Tyr475 overlap spatiallyl | Homo sapiens |
R463L | no major differences between the mutant and the wild-type enzyme for the kinetic parameters of folyl-gamma-L-glutamic acid hydrolysis, a slight decrease in KM values for both N-acetyl-L-aspartyl-L-glutamate and folyl-(gamma-L-glutamic acid)2, folyl-(gamma-L-glutamic acid)3, folyl-(gamma-L-glutamic acid)4 | Homo sapiens |
R511L | no major differences between the mutant and the wild-type enzyme for the kinetic parameters of folyl-gamma-L-glutamic acid hydrolysis, a slight decrease in KM values for both N-acetyl-L-aspartyl-L-glutamate and folyl-(gamma-L-glutamic acid)2, folyl-(gamma-L-glutamic acid)3, folyl-(gamma-L-glutamic acid)4 | Homo sapiens |
W541A | no major differences between the mutant and the wild-type enzyme for the kinetic parameters of folyl-gamma-L-glutamic acid hydrolysis, a slight decrease in KM values for both N-acetyl-L-aspartyl-L-glutamate and folyl-(gamma-L-glutamic acid)2, folyl-(gamma-L-glutamic acid)3, folyl-(gamma-L-glutamic acid)4 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q04609 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
folyl-(gamma-L-glutamic acid)2 + H2O | - |
Homo sapiens | folyl-gamma-L-glutamic acid + L-glutamate | - |
? | |
folyl-(gamma-L-glutamic acid)3 + H2O | - |
Homo sapiens | folyl-(gamma-L-glutamic acid)2 + L-glutamate | - |
? | |
folyl-(gamma-L-glutamic acid)4 + H2O | - |
Homo sapiens | folyl-(gamma-L-glutamic acid)3 + L-glutamate | - |
? | |
folyl-gamma-L-glutamic acid + H2O | - |
Homo sapiens | folate + L-glutamate | - |
? | |
N-acetyl-L-aspartyl-L-glutamate + H2O | - |
Homo sapiens | N-acetyl-L-aspartate + L-glutamate | - |
? |