Crystallization (Comment) | Organism |
---|---|
GCPIII ectodomain in a pseudo-unliganded state and in a complex with product L-glutamate, or the phosphapeptide transition state mimetic (2S,3'S)-[[(3'-amino-3'-carboxy-propyl)-hydroxyphosphinoyl]methyl]-pentanedioic acid, or quisqualic acid, a glutamate biostere, X-ray diffraction structure determination and analysis at 1.29-1.56 A resolution, modelling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,3'S)-[[(3'-amino-3'-carboxy-propyl)-hydroxyphosphinoyl]methyl]-pentanedioic acid | EPE, a phosphapeptide transition state analog of glutamyl-glutamate | Homo sapiens | |
2-amino-3-(3,5-dioxo[1,2,4]oxadiazolidin-2-yl)propionic acid | i.e. quisqualic acid, a glutamate-like inhibitor of GCPIII and GCPII | Homo sapiens | |
L-Glu | - |
Homo sapiens | |
MOPS | - |
Homo sapiens | |
additional information | GCPIII enzyme-inhibitor interactions and the architecture of the S1' pocket, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | GCPII | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | GCPIII and GCPII are metalloenzymes | Homo sapiens | |
Zn2+ | binding structure of Zn2+ ions in the bimetallic active site, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-aspartyl-L-glutamate + H2O | Homo sapiens | - |
L-glutamate + N-acetyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nervous system | GCPII | Homo sapiens | - |
prostate | GCPII | Homo sapiens | - |
small intestine | GCPII | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the S1' pocket, the pharmacophore pocket of GCPIII, is shaped by residues Phe199, Arg200, Asn247, Glu414, Gly417, Leu418, Gly508, Tyr542, Lys689 and Tyr690. The specificity of GCPIII towards the P1' glutamate (or glutamate-like moieties) is determined by a combination of ionic and polar interactionsGCPIII substrate-binding cavity structure, overview | Homo sapiens | ? | - |
? | |
N-acetyl-L-aspartyl-L-glutamate + H2O | - |
Homo sapiens | L-glutamate + N-acetyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | overall fold and quaternary arrangement of the GCPIII molecule, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GCPII | - |
Homo sapiens |
GCPIII | - |
Homo sapiens |
glutamate carboxypeptidase II | - |
Homo sapiens |
glutamate carboxypeptidase III | - |
Homo sapiens |
More | glutamate carboxypeptidase III and glutamate carboxypeptidase II belong to the transferrin receptor/glutamate carboxypeptidase II superfamily | Homo sapiens |
NAALADase | - |
Homo sapiens |
prostate specific membrane antigen | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000129 | - |
(2S,3'S)-[[(3'-amino-3'-carboxy-propyl)-hydroxyphosphinoyl]methyl]-pentanedioic acid | GCPII | Homo sapiens | |
0.0000346 | - |
(2S,3'S)-[[(3'-amino-3'-carboxy-propyl)-hydroxyphosphinoyl]methyl]-pentanedioic acid | GCPIII | Homo sapiens | |
0.00023 | - |
2-amino-3-(3,5-dioxo[1,2,4]oxadiazolidin-2-yl)propionic acid | GCPIII | Homo sapiens | |
0.00102 | - |
2-amino-3-(3,5-dioxo[1,2,4]oxadiazolidin-2-yl)propionic acid | GCPII | Homo sapiens | |
0.27 | - |
L-Glu | GCPIII | Homo sapiens | |
0.428 | - |
L-Glu | GCPII | Homo sapiens |