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Literature summary for 3.4.17.21 extracted from

  • Bzdega, T.; Crowe, S.L.; Ramadan, E.R.; Sciarretta, K.H.; Olszewski, R.T.; Ojeifo, O.A.; Rafalski, V.A.; Wroblewska, B.; Neale, J.H.
    The cloning and characterization of a second brain enzyme with NAAG peptidase activity (2004), J. Neurochem., 89, 627-635.
    View publication on PubMed
Search for more literature for 3.4.17.21

Cloned(Commentary)

Cloned (Comment) Organism
gene Naalad2, DNA and amino acid sequence determination and analysis, expression of GCPIII in CHO cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information GCPII knockout mutant mice do not show an altered phenotype Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
2-(phosphonomethyl)-pentanedioic acid 2-PMPA, GCPIII IC50: 94 nM, GCPII IC50: 6.74 nM Mus musculus
EDTA
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Mus musculus
-
-
membrane
-
 Mus musculus 16020
-
synaptic cleft
-
 Mus musculus 43083
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates the metallopeptidase Mus musculus
Zn2+ stimulates the metallopeptidase Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O Mus musculus the enzyme inactivates the neurotransmitter in the synaptic cleft N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9CZR2 gene Naalad2
-

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte
-
 Mus musculus
-
brain GCPII Mus musculus
-
cerebellum
-
 Mus musculus
-
glial cell GCPII, not GCPIII Mus musculus
-
hippocampus
-
 Mus musculus
-
lung GCPIII Mus musculus
-
additional information tissue distribution of GCPIII activity and expression, overview Mus musculus
-
ovary GCPIII Mus musculus
-
spinal cord
-
 Mus musculus
-
testis GCPIII Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O the enzyme inactivates the neurotransmitter in the synaptic cleft Mus musculus N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O i.e. NAAG Mus musculus N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GCPII
-
 Mus musculus
GCPIII
-
 Mus musculus
glutamate carboxypeptidase III
-
 Mus musculus
N-Acetylated-alpha-linked acidic dipeptidase
-
 Mus musculus
N-acetylated-alpha-linked acidic dipeptidase 2
-
 Mus musculus
N-acetylated-alpha-linked acidic dipeptidase II
-
 Mus musculus
NAAG peptidase
-
 Mus musculus
NAAG peptidase II
-
 Mus musculus
NAALADase
-
 Mus musculus
NAALADase II
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000674
-
 2-PMPA, GCPIII IC50: 94 nM, GCPII IC50: 6.74 nM Mus musculus 2-(phosphonomethyl)-pentanedioic acid
0.000094
-
 2-PMPA, GCPIII IC50: 94 nM, GCPII IC50: 6.74 nM Mus musculus 2-(phosphonomethyl)-pentanedioic acid