Literature summary for 3.4.17.21 extracted from

Meighan, M.A.; Dickerson, M.T.; Glinskii, O.; Glinsky, V.V.; Wright, G.L., Jr.; Deutscher, S.L.
Recombinant glutamate carboxypeptidase II (prostate specific membrane antigen-PSMA)-cellular localization and bioactivity analyses (2003), J. Protein Chem., 22, 317-326.

Search for more literature for 3.4.17.21

Application

Application	Comment	Organism
medicine	the enzyme is an important marker in the diagnosis of prostate cancer	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the full-length enzyme and the mutant enzymes DEALTA20-42 and DELTA1-42 in HEK293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA1-42	the recombinantly expressed extracellular domain has a cytosolic localization compared to sell surface membrane localization of the full-length enzyme. Less than 5% of the activity of the wild-type enzyme	Homo sapiens
DELTA20-42	mutant enzyme omitting the transmembrane domain is associated with the cell surface membrane as is the full-length enzyme. Less than 5% of the activity of the wild-type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	the enzyme is associated with the cell surface membrane. The recombinant enzyme and the mutant enzyme omitting the transmembrane domain is associated with the cell surface membrane, the recombinantly expressed extracellular domain has a cytosolic localization	Homo sapiens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
FLAG affinity chromatography, full-length enzyme and mutant enzymes DELTA1-42 and DELTA20-42	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
LNCaP cell	prostate cancer cell line	Homo sapiens	-

Subunits

Subunits	Comment	Organism
?	x * 110000-120000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
prostate specific membrane antigen	-	Homo sapiens
PSMA	-	Homo sapiens

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Release 2023.1 (January 2023)