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Literature summary for 3.4.17.20 extracted from

  • Sanglas, L.; Valnickova, Z.; Arolas, J.L.; Pallares, I.; Guevara, T.; Sola, M.; Kristensen, T.; Enghild, J.J.; Aviles, F.X.; Gomis-Rueth, F.X.
    Structure of activated thrombin-activatable fibrinolysis inhibitor, a molecular link between coagulation and fibrinolysis (2008), Mol. Cell, 31, 598-606.
    View publication on PubMed
Search for more literature for 3.4.17.20

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Homo sapiens
using the sitting-drop vapor diffusion method. TAFIa conforms to the alpha/beta-hydrolase fold of metallocarboxypeptidases and displays two unique flexible loops on the molecular surface, accounting for structural instability and susceptibility to proteolysis Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
tick carboxypeptidase inhibitor proteinaceous inhibitor TCI which is found in in the hematophagous ixodid tick Rhipicephalus bursa consists of tandem structurally similar small modules, an N-terminal and a C-terminal domain. TCI inhibits TFAIa mainly through its C-terminal domain. The C-terminal residue His75 is cleaved and the new C-terminus Leu74 approaches and contacts the TAFIa catalytic zinc ion through its carboxylate atoms. In addition to this contact, the C-terminal domain of TCI itneracts with TFAIa through 15 hydrogen bonds and 5 hydrophobic contacts Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.38
-
 hippuryl-Arg Vmax: 44.86 micromol/l/min Homo sapiens
3.44
-
 hippuryl-Arg Vmax: 46.05 micromol/l/min Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens TFAI binds heparin ?
-
 ?
additional information Bos taurus TFAI binds heparin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus Q2KIG3
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
purified from plasma using a ECH-lysine sepharose column and anion-exchange chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Bos taurus
-
blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hippuryl-Arg + H2O
-
 Homo sapiens hippuric acid + Arg
-
 ?
hippuryl-Arg + H2O
-
 Bos taurus hippuric acid + Arg
-
 ?
additional information TFAI binds heparin Homo sapiens ?
-
 ?
additional information TFAI binds heparin Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
TAFIa
-
 Homo sapiens
TAFIa
-
 Bos taurus
thrombin-activatable fibrinolysis inhibitor
-
 Homo sapiens
thrombin-activatable fibrinolysis inhibitor
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.67
-
 hippuryl-Arg
-
 Homo sapiens
2.74
-
 hippuryl-Arg
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Bos taurus