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Literature summary for 3.4.17.20 extracted from

  • Do, Y.H.; Gifford-Moore, D.S.; Beight, D.W.; Rathnachalam, R.; Klimkowski, V.J.; Warshawsky, A.M.; Lu, D.
    Inhibition of thrombin activatable fibrinolysis inhibitor by cysteine derivatives (2005), Thromb. Res., 116, 265-271.
    View publication on PubMed
Search for more literature for 3.4.17.20

Inhibitors

Inhibitors Comment Organism Structure
DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid i.e. Plummer's inhibitor, IC50: 0.029 mM Homo sapiens
glycyl-glycyl-L-cysteine
-
 Homo sapiens
glycyl-L-cysteine
-
 Homo sapiens
guanidinyl-L-cysteine IC50: 0.0094 mM in inhibition of plasma fibrin clot lysis Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrin + H2O Homo sapiens partially degraded fibrin in fibrin clots, cleavage of C-terminal Lys fibrin + Lys
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification Ca2+-dependent activation by thrombin at room temperature Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
release of C-terminal Arg and Lys from a polypeptide catalytically important residues are G207, I243, I247, S254, and I255, catalytic mechanism and substrate binding Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrin + H2O partially degraded fibrin in fibrin clots, cleavage of C-terminal Lys Homo sapiens fibrin + Lys
-
 ?
[3-(2-furylacryloyl)]-L-alanyl-L-arginine + H2O
-
 Homo sapiens [3-(2-furylacryloyl)]-L-alanine + L-arginine
-
 ?
[3-(2-furylacryloyl)]-L-phenylalanyl-L-phenylalanine + H2O
-
 Homo sapiens [3-(2-furylacryloyl)]-L-phenylalanine + L-phenylalanine
-
 ?

Subunits

Subunits Comment Organism
More active site ligand complex model building using D-Cys, guanidinyl-L-cysteine, glycyl-glycyl-L-cysteine, and glycyl-L-cysteine Homo sapiens

Synonyms

Synonyms Comment Organism
TAFI
-
 Homo sapiens
thrombin activatable fibrinolysis inhibitor
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperatur Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Homo sapiens
0.00008
-
 guanidinyl-L-cysteine 22°C Homo sapiens
0.0001
-
 DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid 22°C Homo sapiens
0.00014
-
 glycyl-L-cysteine 22°C Homo sapiens
0.00099
-
 glycyl-glycyl-L-cysteine 22°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0094
-
 IC50: 0.0094 mM in inhibition of plasma fibrin clot lysis Homo sapiens guanidinyl-L-cysteine
0.029
-
 i.e. Plummer's inhibitor, IC50: 0.029 mM Homo sapiens DL-mercaptomethyl-3-guanidinoethylthiopropanoic acid