Protein Variants | Comment | Organism |
---|---|---|
S305C/T329I | generation of a stable activated thrombin activable fibrinolysis inhibitor variant by site-directed mutagenesis, the mutant's half-life is 11fold increased compared to the wild-type enzyme, the mutant also shows about 3fold higher fibrin clot lysis activityoverview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the activated enzyme shows anti-fibrinolytic and fibrin clot lysis activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
wild-type isozymes A147/T325 and A147/I325 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation of TAFI to TAFIa by cleavage through thrombin, activation reaction kinetics with wild-type isozymes and mutant enzymes at 37°C and pH 7.4 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-L-arginine + H2O | - |
Homo sapiens | hippuric acid + L-arginine | - |
? | |
additional information | the activated enzyme shows anti-fibrinolytic and fibrin clot lysis activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
activated thrombin activable fibrinolysis inhibitor | - |
Homo sapiens |
TAFI | - |
Homo sapiens |
TAFIa | - |
Homo sapiens |
thrombin activable fibrinolysis inhibitor | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the activated enzyme is thermolabile and inactivated through a conformational change | Homo sapiens |
37 | - |
half-life of wild-type TAFIa is 9.4 min, half-life of TAFIa mutant S305C/T329I is 70 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |