Cloned (Comment) | Organism |
---|---|
expression in BHK cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D87A | activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin. Increase in activation by plasmin. Thermal inactivation at 37°C is similar to that of the wild-type enzyme | Homo sapiens |
additional information | mutant with the fibrinogen Bbeta thrombin cleavage site Ala-Arg-Gly-His-Arg substituted into positions 91-95: activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin, activated by plasmin with the same kinetics as wild-type enzyme. Mutant enzyme with the protein C thrombin cleavage site Asp-Pro-Arg-Leu-Ile-Asp substituted into positions 90-95: no measurable activation by plasmin | Homo sapiens |
S90A | activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin. Increase in activation by plasmin. Thermal inactivation at 37°C is similar to that of the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.979 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
1.089 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
1.11 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
1.26 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibrin + H2O | Homo sapiens | attenuates clot lysis by removing lysine residues from a fibrin clot | Lys + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | - |
variant A147/I325 | - |
Homo sapiens | - |
variant A147/T325 | - |
Homo sapiens | - |
variant T147/I325 | - |
Homo sapiens | - |
variant T147/T325 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of throbin is 0.74 per s, the Km-value is 0.00067 mM | Homo sapiens |
proteolytic modification | the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of throbin is 1.06 per s, the Km-value is 0.00084 mM | Homo sapiens |
proteolytic modification | the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of thrombin is 0.74 per s, the Km-value is 0.00082 mM | Homo sapiens |
proteolytic modification | the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of thrombin is 0.85 per s, the Km-value is 0.00083 mM | Homo sapiens |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anisylazoformyl-Lys + H2O | - |
Homo sapiens | anisylazoformic acid + Lys | - |
? | |
fibrin + H2O | attenuates clot lysis by removing lysine residues from a fibrin clot | Homo sapiens | Lys + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TAFI | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
half-life: 147 min | Homo sapiens |
25 | - |
half-life: 162 min | Homo sapiens |
25 | - |
half-life: 73.8 min | Homo sapiens |
25 | - |
half-life: 77.2 min | Homo sapiens |
30 | - |
half-life: 54 min | Homo sapiens |
30 | - |
half-life: 28.3 min | Homo sapiens |
30 | - |
half-life: 28.6 min | Homo sapiens |
33 | - |
half-life: 14.5 min | Homo sapiens |
33 | - |
half-life: 15.4 min | Homo sapiens |
33 | - |
half-life: 27.2 min | Homo sapiens |
33 | - |
half-life: 27.4 min | Homo sapiens |
37 | - |
half-life: 15 min | Homo sapiens |
37 | - |
half-life: 14.7 min | Homo sapiens |
37 | - |
half-life: 7.8 min | Homo sapiens |
37 | - |
half-life: 7.8 min for wild-type enzyme, 7.6 min for mutant enzyme S90A and 7.5 min for mutant enzyme D87A | Homo sapiens |
37 | - |
half-life: 8.0 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.9 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
26.8 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
27 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
27.4 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens | |
29.5 | - |
anisylazoformyl-Lys | 24°C | Homo sapiens |