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Literature summary for 3.4.17.2 extracted from

  • Raz, A.; Dinparast Djadid, N.; Zakeri, S.
    Molecular characterization of the carboxypeptidase B1 of Anopheles stephensi and its evaluation as a target for transmission-blocking vaccines (2013), Infect. Immun., 81, 2206-2216.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine cpbAs1 can be introduced as a transmission-blocking vaccine candidate in regions where Anopheles stephensi is the main malaria vector Anopheles stephensi

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Anopheles stephensi

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Anopheles stephensi
2-Guanidinoethylmercaptosuccinic acid
-
Anopheles stephensi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.22
-
hippuryl-L-Arg pH 8, 37°C Anopheles stephensi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48100
-
calculated from cDNA Anopheles stephensi

Organism

Organism UniProt Comment Textmining
Anopheles stephensi F1ALX3
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Anopheles stephensi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2757
-
substrate: hippuryl-L-Lys, pH 8, 37°C Anopheles stephensi
15200
-
substrate: hippuryl-L-Arg, pH 8, 37°C Anopheles stephensi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hippuryl-L-Arg
-
Anopheles stephensi hippuric acid + L-Arg
-
?
hippuryl-L-Lys
-
Anopheles stephensi hippuric acid + L-Lys
-
?

Synonyms

Synonyms Comment Organism
Carboxypeptidase B1
-
Anopheles stephensi
cpbAs1
-
Anopheles stephensi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Anopheles stephensi

pH Range

pH Minimum pH Maximum Comment Organism
8
-
assay at Anopheles stephensi

Expression

Organism Comment Expression
Anopheles stephensi decreased expression after blood-feeding in the non-infected group down
Anopheles stephensi expression level is increased after ingestion of the mature gametocytes of Plasmodium falciparum up

General Information

General Information Comment Organism
malfunction anti-CPBAs1 directed antibodies can significantly reduce the mosquito infection rate in the test group compared with the control group Anopheles stephensi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5488
-
hippuryl-L-Arg pH 8, 37°C Anopheles stephensi