Application | Comment | Organism |
---|---|---|
medicine | cpbAs1 can be introduced as a transmission-blocking vaccine candidate in regions where Anopheles stephensi is the main malaria vector | Anopheles stephensi |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Anopheles stephensi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Anopheles stephensi | |
2-Guanidinoethylmercaptosuccinic acid | - |
Anopheles stephensi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.22 | - |
hippuryl-L-Arg | pH 8, 37°C | Anopheles stephensi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48100 | - |
calculated from cDNA | Anopheles stephensi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles stephensi | F1ALX3 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Anopheles stephensi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2757 | - |
substrate: hippuryl-L-Lys, pH 8, 37°C | Anopheles stephensi |
15200 | - |
substrate: hippuryl-L-Arg, pH 8, 37°C | Anopheles stephensi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-L-Arg | - |
Anopheles stephensi | hippuric acid + L-Arg | - |
? | |
hippuryl-L-Lys | - |
Anopheles stephensi | hippuric acid + L-Lys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Carboxypeptidase B1 | - |
Anopheles stephensi |
cpbAs1 | - |
Anopheles stephensi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Anopheles stephensi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Anopheles stephensi |
Organism | Comment | Expression |
---|---|---|
Anopheles stephensi | decreased expression after blood-feeding in the non-infected group | down |
Anopheles stephensi | expression level is increased after ingestion of the mature gametocytes of Plasmodium falciparum | up |
General Information | Comment | Organism |
---|---|---|
malfunction | anti-CPBAs1 directed antibodies can significantly reduce the mosquito infection rate in the test group compared with the control group | Anopheles stephensi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5488 | - |
hippuryl-L-Arg | pH 8, 37°C | Anopheles stephensi |