Literature summary for 3.4.17.2 extracted from

Marinkovic, D.V.; Marinkovic, J.N.; Erd�s, E.G.; Robinson, C.J.G.
Purification of carboxypeptidase B from human pancreas (1977), Biochem. J., 163, 253-260.

Search for more literature for 3.4.17.2

Inhibitors

Inhibitors	Comment	Organism	Structure
6-amino-n-hexanoic acid	-	Homo sapiens
Co2+	hydrolysis of hippuryl-L-Arg	Homo sapiens
EDTA	partial	Homo sapiens
SDS	-	Homo sapiens
Urea	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	hippuryl-L-Arg	carboxypeptidase B I	Homo sapiens
0.071	-	hippuryl-L-Arg	carboxypeptidase B II	Homo sapiens
0.277	-	hippuryl-Arg	carboxypeptidase B I	Homo sapiens
0.31	-	hippuryl-Arg	carboxypeptidase B II	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	CoCl2, accelerates hydrolysis of hippuryl-L-Arg and hippuryl-Lys	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32210	-	carboxypeptidase B II, gel filtration	Homo sapiens
32920	-	carboxypeptidase B I, gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
862	-	carboxypeptidase B I	Homo sapiens
1192	-	carboxypeptidase B II	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuryl-L-Arg	-	Homo sapiens	hippuric acid + L-Arg	-	?
hippuryl-Lys + H2O	peptidase activity	Homo sapiens	hippuric acid + Lys	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	cleavage of hippuryl-L-Arg	Homo sapiens
10	-	hydrolysis of hippuryl-L-Arg	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	pH 6: about 60% of maximal activity with carboxypeptidase B I, about 50% of maximal activity with carboxypeptidase B II, pH 10: about 70% of maximal activity with carboxypeptidase B I, about 60% of maximal activity with carboxypeptidase B II, cleavage of hippuryl-L-Arg	Homo sapiens
7	10.5	pH 7: about 45% of maximal activity with carboxypeptidase B I, about 30% of maximal activity with carboxypeptidase B II, pH: 10.5: about 90% of maximal activity with carboxypeptidase B I, about 80% of maximal activity with carboxypeptidase B II, hydrolysis of hippuryl-L-Arg	Homo sapiens

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Release 2023.1 (January 2023)