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Literature summary for 3.4.17.15 extracted from

  • Reverter, D.; Garcia-Saez, I.; Catasus, L.; Vendrell, J.; Coll, M.; Aviles, F.X.
    Characterization and preliminary x-ray diffraction analysis of human pancreatic procarboxypeptidase A2 (1997), FEBS Lett., 420, 7-10.
    View publication on PubMed
Search for more literature for 3.4.17.15

Cloned(Commentary)

Cloned (Comment) Organism
procarboxypeptidase A2 is expressed in Pichia pastoris Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method at 4°C and at 20°C, procarboxypeptidase A2 is crystallized using vapour diffusion approach. The crystals belong to the monoclinic system spacegroup P21 and present one procarboxypeptidase A2 molecule per asymmetric unit Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 benzyloxycarbonyl-Gly-Gly-Tyr pH 7.5, 25°C Homo sapiens
0.146
-
 benzyloxycarbonyl-Gly-Gly-Trp pH 7.5, 25°C Homo sapiens
0.175
-
 Benzyloxycarbonyl-Gly-Tyr pH 7.5, 25°C Homo sapiens
0.261
-
 benzyloxycarbonyl-Gly-Trp pH 7.5, 25°C Homo sapiens
0.372
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens
2.028
-
 benzyloxycarbonyl-Gly-Trp pH 7.5, 25°C Homo sapiens
2.27
-
 Benzyloxycarbonyl-Gly-Phe pH 7.5, 25°C Homo sapiens
5.3
-
 benzyloxycarbonyl-Gly-Gly-Leu pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification synthesized as procarboxypeptidase A2 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-Leu + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Phe + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Trp + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Tyr + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Phe + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Trp + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-Gly-Tyr + H2O
-
 Homo sapiens ?
-
 ?
additional information no measurable activity with benzyloxycarbonyl-Gly-Gly-Val, benzyloxycarbonyl-Gly-Gly-Ala und benzyloxycarbonyl-Gly-Gly-Ser. CPA2 is the only reported carboxypeptidase with specificity for C-terminal Trp residues Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.7
-
 Benzyloxycarbonyl-Gly-Tyr pH 7.5, 25°C Homo sapiens
11.8
-
 benzyloxycarbonyl-Gly-Gly-Leu pH 7.5, 25°C Homo sapiens
16.1
-
 Benzyloxycarbonyl-Gly-Phe pH 7.5, 25°C Homo sapiens
58.1
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens
70
-
 benzyloxycarbonyl-Gly-Gly-Tyr pH 7.5, 25°C Homo sapiens
90.3
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens